The Mouse GT-34 (Gastrin-34) CLIA Kit is a reliable tool for the accurate measurement of GT-34 levels in mouse serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit provides consistent and reproducible results, making it suitable for various research applications.GT-34, also known as Gastrin-34, is a peptide hormone that plays a crucial role in regulating gastric acid secretion and promoting the growth of gastric mucosa.
Dysregulation of GT-34 levels has been associated with gastrointestinal disorders such as gastric ulcers and gastrinomas. By detecting GT-34 levels, researchers can gain insights into these conditions and potentially develop new therapeutic strategies.Overall, the Mouse GT-34 CLIA Kit is a valuable tool for studying the role of GT-34 in gastric physiology and pathology, offering researchers a reliable method for quantifying GT-34 levels in mouse samples.
Product Name:
Mouse GT-34 (Gastrin 34) CLIA Kit
SKU:
MOES00285
Target:
Mouse GT-34 (Gastrin 34)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse GT-34. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse GT-34 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GT-34, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse GT-34. You can calculate the concentration of Mouse GT-34 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-113
98-111
95-106
Average (%)
103
105
100
1:4
Range (%)
98-112
102-114
87-100
Average (%)
103
108
92
1:8
Range (%)
91-108
98-111
100-114
Average (%)
99
105
107
1:16
Range (%)
89-103
85-100
93-107
Average (%)
94
91
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-100
92
EDTA plasma (n=5)
96-111
104
Cell culture media (n=5)
89-102
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
25.57
73.02
217.54
23.2
71.03
199.68
Standard deviation
2.16
7.46
24.67
2.74
8.25
16.47
C V (%)
8.45
10.22
11.34
11.81
11.61
8.25
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse GT-34 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse GT-34 in samples. No significant cross-reactivity or interference between Mouse GT-34 and analogues was observed.
