The Mouse GT Gastrin ELISA Kit is specifically developed for the precise measurement of GT Gastrin levels in mouse samples. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results, making it suitable for various research purposes. GT Gastrin is a key hormone involved in regulating gastric acid secretion and stimulating cell growth in the stomach lining. It plays a crucial role in gastrointestinal health and is implicated in conditions such as peptic ulcers and gastrinomas. Studying GT Gastrin levels can provide valuable insights into these conditions and aid in the development of potential treatments. With its reliable performance and ease of use, the Mouse GT Gastrin ELISA Kit is an essential tool for researchers studying the role of GT Gastrin in gastric physiology and associated disorders.
Product Name:
Mouse GT (Gastrin) ELISA Kit
SKU:
MOES01804
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.