The Mouse MIF (Macrophage Migration Inhibitory Factor) ELISA Kit is a powerful tool for the quantification of MIF levels in mouse serum, plasma, and tissue lysates. With its high sensitivity and specificity, this kit provides accurate and reliable results for a variety of research applications.MIF is a key cytokine involved in the regulation of innate and adaptive immune responses, as well as inflammation and tissue repair processes. This protein has been implicated in various diseases such as autoimmune disorders, infectious diseases, and cancer, making it a valuable target for therapeutic interventions and biomarker studies.Overall, the Mouse MIF ELISA Kit offers researchers a comprehensive solution for studying the role of MIF in health and disease, providing valuable insights into potential treatments and diagnostics.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
