After thorough analysis of the provided URL, I found that the Mouse Osteonectin CLIA Kit (MOES00464) from AssayGenie is a cutting-edge tool designed for the quantitative measurement of osteonectin levels in mouse serum, plasma, and tissue lysates. This CLIA kit offers unparalleled accuracy and precision, ensuring dependable and consistent results for a wide array of research applications.Osteonectin, also known as SPARC (Secreted Protein Acidic and Rich in Cysteine), is a critical protein involved in bone formation and remodeling, as well as in the regulation of cell-matrix interactions.
Its dysregulation has been implicated in various diseases, such as osteoporosis and cancer, making it a valuable biomarker for studying these conditions and potentially developing therapeutic interventions.With its advanced technology and user-friendly format, the Mouse Osteonectin CLIA Kit from AssayGenie is an indispensable tool for researchers in the field of bone biology and beyond. Experience the convenience and reliability of this innovative kit for your next experimental endeavor.
Product Name:
Mouse ON (Osteonectin) CLIA Kit
SKU:
MOES00464
Target:
Mouse ON (Osteonectin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse ON. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse ON and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ON, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse ON. You can calculate the concentration of Mouse ON in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-105
101-116
93-108
Average (%)
97
109
99
1:4
Range (%)
103-118
93-105
87-101
Average (%)
109
100
94
1:8
Range (%)
87-99
94-108
100-116
Average (%)
94
99
106
1:16
Range (%)
97-113
94-108
86-99
Average (%)
103
100
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-97
92
EDTA plasma (n=5)
89-103
95
Cell culture media (n=5)
97-110
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.19
3.4
14.9
2.34
3.56
16.0
Standard deviation
0.2
0.34
1.28
0.29
0.42
1.02
C V (%)
9.13
10.0
8.59
12.39
11.8
6.38
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse ON concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse ON in samples. No significant cross-reactivity or interference between Mouse ON and analogues was observed.
