The Mouse OXA (Orexin A) ELISA Kit is specifically designed for the precise measurement of orexin A levels in mouse serum, plasma, and tissue homogenates. With its exceptional sensitivity and specificity, this kit delivers consistent and accurate results, making it a valuable tool for various research endeavors.Orexin A is a neuropeptide that plays a crucial role in regulating wakefulness and feeding behavior. Dysregulation of orexin A has been implicated in various disorders, including sleep disorders, obesity, and addiction. Therefore, monitoring orexin A levels can provide valuable insights into these conditions and aid in the development of new therapeutic strategies.Get your Mouse OXA ELISA Kit now and unlock the potential for groundbreaking discoveries in the field of neuroscience and metabolism research.
Product Name:
Mouse OXA (Orexin A) ELISA Kit
SKU:
MOES01327
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
37.5 pg/mL
Detection range:
62.5-4000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
