The Human OXA (Orexin-A) ELISA Kit is specifically designed for the precise measurement of orexin-A levels in human samples such as serum, plasma, and cell culture supernatants. This kit is characterized by its high sensitivity and specificity, ensuring accurate and consistent results, making it an excellent tool for various research applications.Orexin-A, also known as hypocretin-1, is a neuropeptide that plays a crucial role in regulating wakefulness, appetite, and energy metabolism. Dysregulation of orexin-A has been implicated in conditions such as narcolepsy, obesity, and sleep disorders, making it a valuable biomarker for studying these disorders and developing potential interventions.By utilizing the Human OXA ELISA Kit, researchers can gain valuable insights into the role of orexin-A in health and disease, facilitating the advancement of scientific knowledge and the development of novel therapeutic strategies.
Product Name:
Human OXA (Orexin A) ELISA Kit
SKU:
HUES02121
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
37.5 pg/mL
Detection range:
62.5-4000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
