The Bovine Orexin-A (OXA) ELISA Kit is specifically designed for the precise detection of orexin-A levels in bovine serum, plasma, and tissue homogenates. This kit offers superior sensitivity and specificity, guaranteeing accurate and consistent results for various research purposes.Orexin-A is a neuropeptide that regulates sleep-wake cycles, appetite, and energy balance in mammals. Dysregulation of orexin-A has been linked to various conditions, including sleep disorders, obesity, and neurological diseases.
Therefore, measuring orexin-A levels can provide valuable insights into these conditions and aid in the development of potential treatments.With its reliability and efficiency, the Bovine Orexin-A (OXA) ELISA Kit is an indispensable tool for researchers studying the role of orexin-A in bovine physiology and pathology. Get your kit today and unlock the potential of your research.
Product Name:
Bovine Orexin A (OXA) ELISA Kit
Product Code:
BODL00133
Size:
96 Assays
Target:
OXA
Synonyms:
PPOX, Hypocretin-1, Hcrt1
Detection Method:
Competition
Range:
31.25-2,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C
3.
Aspirate and wash 3 times
4.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.
Aspirate and wash 5 times
6.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.
Add 50µL Stop Solution. Read at 450 nm immediately.
