The Mouse SIGA (Secretory Immunoglobulin A) Elisa Kit is a highly reliable and accurate tool for detecting SIGA levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures precise and reproducible results, making it perfect for various research applications.SIGA is a key component of the immune system, playing a crucial role in mucosal immunity and protecting against infections in the respiratory and gastrointestinal tracts. Abnormal levels of SIGA have been associated with conditions such as autoimmune diseases, allergies, and infectious diseases, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.Overall, the Mouse SIGA Elisa Kit offers researchers a powerful tool to study SIGA levels in mouse models, furthering our understanding of immune responses and potential treatment options for a variety of diseases.
Product Name:
Mouse sIgA (Secretory Immunoglobulin A) ELISA Kit
SKU:
MOES01461
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
