Description
Mouse Uba52 Recombinant Protein (N-GST)
Mouse Uba52 Recombinant Protein (N-GST) is a recombinant protein expressed in E. coli, spanning Gln2-Lys128, with an N-terminal GST tag. Predicted molecular weight: 41.42 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Ubiquitin-ribosomal protein eL40 fusion protein (Uba52) is a ~14 kDa protein. Exists either covalently attached to another protein, or free (unanchored). For research use only.
When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in proteotoxic stress response and cell cycle; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed.
| Product Name: | Mouse Uba52 Recombinant Protein (N-GST) |
| SKU: | GXRP07061 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Mouse |
| Synonyms: | Ubiquitin-ribosomal protein eL40 fusion protein, Ubiquitin A-52 residue ribosomal protein fusion product 1, Ubiquitin, Large ribosomal subunit protein eL40, 60S ribosomal protein L40, CEP52, Uba52, Ubcep2 |
| Tag: | N-GST |
| Expression Host: | E. coli |
| Protein Length: | Gln2-Lys128 |
| Calculated MW: | 41.42 kDa |
| UniProt: | P62984 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
