Description
Mouse YTHDF2/HGRG8 Recombinant Protein (N-GST)
Mouse YTHDF2/HGRG8 Recombinant Protein (N-GST) is a recombinant protein expressed in E. coli, spanning Gly410-Lys579, with an N-terminal GST tag. Predicted molecular weight: 46.58 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. YTH domain-containing family protein 2 (YTHDF2/HGRG8) is a ~62 kDa protein. Specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs, and regulates their stability. For research use only.
M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in mRNA stability and processing. Acts as a regulator of mRNA stability by promoting degradation of m6A-containing mRNAs via interaction with the CCR4-NOT and ribonuclease P/MRP complexes, depending on the context. The YTHDF paralogs (YTHDF1, YTHDF2 and YTHDF3) share m6A-containing mRNAs targets and act redundantly to mediate mRNA degradation and cellular differentiation. M6A-containing mRNAs containing a binding site for RIDA/HRSP12 (5'-GGUUC-3') are preferentially degraded by endoribonucleolytic cleavage: cooperative binding of RIDA/HRSP12 and YTHDF2 to transcripts leads to recruitment of the ribonuclease P/MRP complex.
| Product Name: | Mouse YTHDF2/HGRG8 Recombinant Protein (N-GST) |
| SKU: | GXRP07882 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Mouse |
| Synonyms: | YTH domain-containing family protein 2, Ythdf2 |
| Tag: | N-GST |
| Expression Host: | E. coli |
| Protein Length: | Gly410-Lys579 |
| Calculated MW: | 46.58 kDa |
| UniProt: | Q91YT7 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
