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Anti-MPG Antibody (CAB5410)

SKU:
CAB5410
Product Type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
  • Epigenetics and Nuclear Signaling Antibodies 3 Anti-MPG Antibody CAB5410
  • Epigenetics and Nuclear Signaling Antibodies 3 Anti-MPG Antibody CAB5410
  • Epigenetics and Nuclear Signaling Antibodies 3 Anti-MPG Antibody CAB5410
  • Epigenetics and Nuclear Signaling Antibodies 3 Anti-MPG Antibody CAB5410
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Description

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Antibody Name:Anti-MPG Antibody
Antibody SKU:CAB5410
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-293 of human MPG (NP_001015052.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:Human, Mouse
Positive Samples:MCF7, K-562, U-937, Mouse liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-293 of human MPG (NP_001015052.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MPAR SGAQ FCRR MGQK KQRP ARAG QPHS SSDA AQAP AEQP HSSS DAAQ APCP RERC LGPP TTPG PYRS IYFS SPKG HLTR LGLE FFDQ PAVP LARA FLGQ VLVR RLPN GTEL RGRI VETE AYLG PEDE AAHS RGGR QTPR NRGM FMKP GTLY VYII YGMY FCMN ISSQ GDGA CVLL RALE PLEG LETM RQLR STLR KGTA SRVL KDRE LCSG PSKL CQAL AINK SFDQ RDLA QDEA VWLE RGPL EPSE PAVV AAAR VGVG HAGE WARK PLRF YVRG SPWV SVVD RVAE QDTQ A
Gene ID:4350
Uniprot:P29372
Cellular Location:Cytoplasm, Mitochondrion matrix, Nucleus, mitochondrion nucleoid
Calculated MW:30kDa/32kDa
Observed MW:37kDa
Synonyms:MPG, AAG, ADPG, APNG, CRA36.1, MDG, Mid1, PIG11, PIG16, anpg
Background:
UniProt Protein Function:MPG: Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, and 7-methylguanine from the damaged DNA polymer formed by alkylation lesions. Belongs to the DNA glycosylase MPG family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 3.2.2.21; Hydrolase; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: nucleoplasm

Molecular Function:protein binding; alkylbase DNA N-glycosylase activity; DNA-3-methyladenine glycosylase I activity; damaged DNA binding

Biological Process: DNA dealkylation; base-excision repair, AP site formation; depurination; base-excision repair; DNA repair

UniProt Code:P29372
NCBI GenInfo Identifier:206729922
NCBI Gene ID:4350
NCBI Accession:P29372.3
UniProt Secondary Accession:P29372,Q13770, Q15275, Q15961, Q5J9I4, Q96BZ6, Q96S33 Q9NNX5, G5E9E2,
UniProt Related Accession:P29372
Molecular Weight:298
NCBI Full Name:DNA-3-methyladenine glycosylase
NCBI Synonym Full Names:N-methylpurine-DNA glycosylase
NCBI Official Symbol:MPG  
NCBI Official Synonym Symbols:AAG; MDG; ADPG; APNG; Mid1; anpg; PIG11; PIG16; CRA36.1  
NCBI Protein Information:DNA-3-methyladenine glycosylase; 3-alkyladenine DNA glycosylase; 3-methyladenine DNA glycosidase; proliferation-inducing protein 11; proliferation-inducing protein 16; N-methylpurine-DNA glycosylase, MPG; CRA36.1 (3-methyl-adenine DNA glycosylase); 3' end of the Mid1 gene, localized 68 kb upstream the humanzeta globin gene on 16p
UniProt Protein Name:DNA-3-methyladenine glycosylase
UniProt Synonym Protein Names:3-alkyladenine DNA glycosylase; 3-methyladenine DNA glycosidase; ADPG; N-methylpurine-DNA glycosylase
Protein Family:DNA-3-methyladenine glycosylase
UniProt Gene Name:MPG  
UniProt Entry Name:3MG_HUMAN
Anti-MPG Antibody (CAB5410)Western blot analysis of extracts of various cell lines, using MPG antibody (CAB5410) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 40s.
Anti-MPG Antibody (CAB5410)Immunofluorescence analysis of U2OS cells using MPG antibody (CAB5410). Blue: DAPI for nuclear staining.
Anti-MPG Antibody (CAB5410)Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using MPG antibody (CAB5410). Green: GFP-RNF168 fusion protein expression for DNA damage marker. Blue: DAPI for nuclear staining. RNF168 (GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.
Anti-MPG Antibody (CAB5410)Immunofluorescence analysis of A549 cells using MPG antibody (CAB5410).

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