Anti-MPI Antibody (CAB7319)

Antibody Name:	Anti-MPI Antibody
Antibody SKU:	CAB7319
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-423 of human MPI (NP_002426.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	Jurkat, HepG2, HeLa, SW620, Mouse heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-423 of human MPI (NP_002426.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAAP RVFP LSCA VQQY AWGK MGSN SEVA RLLA SSDP LAQI AEDK PYAE LWMG THPR GDAK ILDN RISQ KTLS QWIA ENQD SLGS KVKD TFNG NLPF LFKV LSVE TPLS IQAH PNKE LAEK LHLQ APQH YPDA NHKP EMAI ALTP FQGL CGFR PVEE IVTF LKKV PEFQ FLIG DEAA THLK QTMS HDSQ AVAS SLQS CFSH LMKS EKKV VVEQ LNLL VKRI SQQA AAGN NMED IFGE LLLQ LHQQ YPGD IGCF AIYF LNLL TLKP GEAM FLEA NVPH AYLK GDCV ECMA CSDN TVRA GLTP KFID VPTL CEML SYTP SSSK DRLF LPTR SQED PYLS IYDP PVPD FTIM KTEV PGSV TEYK VLAL DSAS ILLM VQGT VIAS TPTT QTPI PLQR GGVL FIGA NESV SLKL TEPK DLLI FRAC CLL
Gene ID:	4351
Uniprot:	P34949
Cellular Location:	Cytoplasm
Calculated MW:	39kDa/46kDa
Observed MW:	47kDa

Synonyms:

MPI, CDG1B, PMI, PMI1

Background:

Phosphomannose isomerase catalyzes the interconversion of fructose-6-phosphate and mannose-6-phosphate and plays a critical role in maintaining the supply of D-mannose derivatives, which are required for most glycosylation reactions. Mutations in the MPI gene were found in patients with carbohydrate-deficient glycoprotein syndrome, type Ib. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	MPI: Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions. Defects in MPI are the cause of congenital disorder of glycosylation type 1B (CDG1B); also known as carbohydrate-deficient glycoprotein syndrome type Ib (CDGS1B). Congenital disorders of glycosylation are metabolic deficiencies in glycoprotein biosynthesis that usually cause severe mental and psychomotor retardation. They are characterized by under- glycosylated serum glycoproteins. CDG1B is clinically characterized by protein-losing enteropathy. Belongs to the mannose-6-phosphate isomerase type 1 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Carbohydrate Metabolism - fructose and mannose; Carbohydrate Metabolism - amino sugar and nucleotide sugar; EC 5.3.1.8; Isomerase Chromosomal Location of Human Ortholog: 15q24.1 Cellular Component: cytosol Molecular Function:mannose-6-phosphate isomerase activity Biological Process: GDP-mannose biosynthetic process Disease: Congenital Disorder Of Glycosylation, Type Ib
NCBI Summary:	Phosphomannose isomerase catalyzes the interconversion of fructose-6-phosphate and mannose-6-phosphate and plays a critical role in maintaining the supply of D-mannose derivatives, which are required for most glycosylation reactions. Mutations in the MPI gene were found in patients with carbohydrate-deficient glycoprotein syndrome, type Ib. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
UniProt Code:	P34949
NCBI GenInfo Identifier:	462567
NCBI Gene ID:	4351
NCBI Accession:	P34949.2
UniProt Secondary Accession:	P34949,Q96AB0, A8K8K9,
UniProt Related Accession:	P34949
Molecular Weight:	39,834 Da
NCBI Full Name:	Mannose-6-phosphate isomerase
NCBI Synonym Full Names:	mannose phosphate isomerase
NCBI Official Symbol:	MPI
NCBI Official Synonym Symbols:	PMI; PMI1; CDG1B
NCBI Protein Information:	mannose-6-phosphate isomerase
UniProt Protein Name:	Mannose-6-phosphate isomerase
UniProt Synonym Protein Names:	Phosphohexomutase; Phosphomannose isomerase; PMI
Protein Family:	Mannose-6-phosphate isomerase
UniProt Gene Name:	MPI
UniProt Entry Name:	MPI_HUMAN

	Western blot analysis of extracts of various cell lines, using MPI antibody (CAB7319) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded rat heart using MPI antibody (CAB7319) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of MCF7 cells using MPI antibody (CAB7319). Blue: DAPI for nuclear staining.

Antibodies

MPI Antibody (PACO10554)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)