Description
| Product Name: | MPO Peroxidation Activity Assay Kit - Fluorometric |
| Product Code: | MAES0004 |
| Product Size: | 96 Assays |
| Sample Type: | Serum, plasma, animal tissue |
| Assay Time: | 20 min |
| Instrument: | Fluorescence Microplate Reader |
| Sensitivity: | 0.001 U/L |
| Detection Range: | 0.001 - 1.26 U/L |
| Inter CV: | 5.4 |
| Inter CV: | 1 |
Myeloperoxidase is a heme-containing cationic glycoprotein that belongs to the heme peroxidase family in mammals. MPO is a dimer formed by polymerization of two subunits. Each subunit contains a heavy chain and a light chain. MPO is abundant in the azuropathic granules of polymorphonuclear leukocytes (PMNLs) and a small number in monocytes and macrophages. Studies have shown that MPO plays an important role in the generation of oxidants and host defense in neutrophils and is closely related to the pathogenesis of many diseases, including cardiovascular disease, lung injury and cancer.
Under the catalysis of peroxidase, hydrogen peroxide can oxidize the non-fluorescent probe into the fluorescent substance, and its fluorescence intensity is proportional to the total peroxidase activity in the sample. This kit specifically inhibits the peroxidase activity of MPO in the sample through an MPO enzyme inhibitor, thus distinguishing the peroxidase activity of MPO in the sample from that of other peroxidases.
| Shelf Life: | 6 Months |
| Storage: | -20°C |
| Shipping: | Gel Pack |
| Research Area: | Enzymes, Oxidative stress |
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