Anti-MRPS31 Antibody (CAB12923)

Antibody Name:	Anti-MRPS31 Antibody
Antibody SKU:	CAB12923
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 66-395 of human MRPS31 (NP_005821.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:100 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Rat
Positive Samples:	Jurkat, HeLa, HepG2, MCF7, A-431

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 66-395 of human MRPS31 (NP_005821.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	ICSK KDKQ SVRT EETS KETS ESQD SEKE NTKK DLLG IIKG MKVE LSTV NVRT TKPP KRRP LKSL EATL GRLR RATE YAPK KRIE PLSP ELVA AASA VADS LPFD KQTT KSEL LSQL QQHE EESR AQRD AKRP KISF SNII SDMK VARS ATAR VRSR PELR IQFD EGYD NYPG QEKT DDLK KRKN IFTG KRLN IFDM MAVT KEAP ETDT SPSL WDVE FAKQ LATV NEQP LQNG FEEL IQWT KEGK LWEF PINN EAGF DDDG SEFH EHIF LEKH LESF PKQG PIRH FMEL VTCG LSKN PYLS VKQK VEHI EWFR NYFN EKKD ILKE SNIQ FN
Gene ID:	10240
Uniprot:	Q92665
Cellular Location:	Mitochondrion
Calculated MW:	45kDa
Observed MW:	37kDa

Synonyms:

MRPS31, IMOGN38, MRP-S31, S31mt

Background:

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. The 28S subunit of the mammalian mitoribosome may play a crucial and characteristic role in translation initiation. This gene encodes a 28S subunit protein that has also been associated with type 1 diabetes; however, its relationship to the etiology of this disease remains to be clarified. Pseudogenes corresponding to this gene have been found on chromosomes 3 and 13.

UniProt Protein Function:	MRPS31: Component of the mitochondrial ribosome small subunit (28S) which comprises a 12S rRNA and about 30 distinct proteins.
UniProt Protein Details:	Protein type:Mitochondrial; RNA-binding Chromosomal Location of Human Ortholog: 13q14.11 Cellular Component: mitochondrial inner membrane; mitochondrion; nucleolus Molecular Function:protein binding; protein domain specific binding
NCBI Summary:	Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. The 28S subunit of the mammalian mitoribosome may play a crucial and characteristic role in translation initiation. This gene encodes a 28S subunit protein that has also been associated with type 1 diabetes; however, its relationship to the etiology of this disease remains to be clarified. Pseudogenes corresponding to this gene have been found on chromosomes 3 and 13. [provided by RefSeq, Jul 2008]
UniProt Code:	Q92665
NCBI GenInfo Identifier:	93186129
NCBI Gene ID:	10240
NCBI Accession:	Q92665.3
UniProt Secondary Accession:	Q92665,Q5VYC8, Q8WTV8, B2RCS3,
UniProt Related Accession:	Q92665
Molecular Weight:	45,318 Da
NCBI Full Name:	28S ribosomal protein S31, mitochondrial
NCBI Synonym Full Names:	mitochondrial ribosomal protein S31
NCBI Official Symbol:	MRPS31
NCBI Official Synonym Symbols:	S31mt; IMOGN38; MRP-S31
NCBI Protein Information:	28S ribosomal protein S31, mitochondrial
UniProt Protein Name:	28S ribosomal protein S31, mitochondrial
UniProt Synonym Protein Names:	Imogen 38
Protein Family:	28S ribosomal protein
UniProt Gene Name:	MRPS31
UniProt Entry Name:	RT31_HUMAN

	Western blot analysis of extracts of various cell lines, using MRPS31 antibody (CAB12923) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Confocal immunofluorescence analysis of U2OS cells using MRPS31 Polyclonal Antibody (CAB12923) at dilution of 1:200. Blue: DAPI for nuclear staining.

Antibodies

MRPS31 Antibody (PACO10613)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)