Anti-MRPS35 Antibody (CAB15878)

Antibody Name:	Anti-MRPS35 Antibody
Antibody SKU:	CAB15878
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-323 of human MRPS35 (NP_068593.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	HT-29, A-549, BxPC-3, A-375, Mouse heart, Mouse kidney, Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-323 of human MRPS35 (NP_068593.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAAA ALPA WLSL QSRA RTLR AFST AVYS ATPV PTPS LPER TPGN ERPP RRKA LPPR TEKM AVDQ DWPS VYPV AAPF KPSA VPLP VRMG YPVK KGVP MAKE GNLE LLKI PNFL HLTP VAIK KHCE ALKD FCTE WPAA LDSD EKCE KHFP IEID STDY VSSG PSVR NPRA RVVV LRVK LSSL NLDD HAKK KLIK LVGE RYCK TTDV LTIK TDRC PLRR QNYD YAVY LLTV LYHE SWNT EEWE KSKT EADM EEYI WENS SSER NILE TLLQ MKAA EKNM EINK EELL GTKE IEEY KKSV VSLK NEEE NENS ISQY KESV KRLL NVT
Gene ID:	60488
Uniprot:	P82673
Cellular Location:	Mitochondrion
Calculated MW:	21kDa/36kDa
Observed MW:	37kDa

Synonyms:

MRPS35, HDCMD11P, MDS023, MRP-S28, MRPS28

Background:

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that has had confusing nomenclature in the literature. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Pseudogenes corresponding to this gene are found on chromosomes 3p, 5q, and 10q.

UniProt Protein Function:	TMEPAI: Involved in down-regulation of the androgen receptor (AR). Enhances ubiquitination and proteasome-mediated degradation of AR, probably by recruiting NEDD4. Belongs to the PMEPA1 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 20q13.31-q13.33 Cellular Component: Golgi membrane; early endosome membrane; integral to membrane; plasma membrane; endosome membrane Molecular Function:protein binding; WW domain binding Biological Process: transforming growth factor beta receptor signaling pathway; androgen receptor signaling pathway; negative regulation of transforming growth factor beta receptor signaling pathway
NCBI Summary:	This gene encodes a transmembrane protein that contains a Smad interacting motif (SIM). Expression of this gene is induced by androgens and transforming growth factor beta, and the encoded protein suppresses the androgen receptor and transforming growth factor beta signaling pathways though interactions with Smad proteins. Overexpression of this gene may play a role in multiple types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]
UniProt Code:	P82673
NCBI GenInfo Identifier:	364023812
NCBI Gene ID:	56937
NCBI Accession:	NP_001242905.1
UniProt Secondary Accession:	P82673,Q5TDR6, Q8NER4, Q96B72, Q9UJD3,
UniProt Related Accession:	Q969W9
Molecular Weight:
NCBI Full Name:	protein TMEPAI isoform d
NCBI Synonym Full Names:	prostate transmembrane protein, androgen induced 1
NCBI Official Symbol:	PMEPA1
NCBI Official Synonym Symbols:	STAG1; TMEPAI
NCBI Protein Information:	protein TMEPAI; solid tumor-associated 1 protein; transmembrane, prostate androgen induced RNA; prostate transmembrane protein androgen induced 1
UniProt Protein Name:	Protein TMEPAI
UniProt Synonym Protein Names:	Prostate transmembrane protein androgen induced 1; Solid tumor-associated 1 protein; Transmembrane prostate androgen-induced protein
Protein Family:	Protein
UniProt Gene Name:	PMEPA1
UniProt Entry Name:	PMEPA_HUMAN

	Western blot analysis of extracts of various cell lines, using MRPS35 antibody (CAB15878) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunohistochemistry of paraffin-embedded Human colon carcinoma using MRPS35 Rabbit pAb (CAB15878) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of L929 cells using MRPS35 antibody (CAB15878) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

MRPS35 Antibody (PACO06658)

MRPS35 Antibody (PACO10615)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)