The Mouse G-CSF (Granulocyte Colony-Stimulating Factor) ELISA Kit - High Sensitivity is a cutting-edge assay meticulously designed to quantitatively measure levels of Granulocyte Colony-Stimulating Factor (G-CSF) in various biological samples from mouse models. G-CSF is a pivotal cytokine responsible for the stimulation of granulocyte production and plays a crucial role in immune responses, hematopoiesis, and inflammatory processes. By accurately detecting G-CSF levels with high sensitivity, this ELISA kit enables researchers to gain insights into the dynamic regulation of granulocyte homeostasis and immune function. This high-sensitivity ELISA kit empowers researchers to explore the intricate mechanisms of G-CSF signaling, providing a deeper understanding of its impact on immune cell development, proliferation, and mobilization. By measuring G-CSF levels in mouse samples, researchers can unravel the complexities of hematopoiesis, immune modulation, and inflammatory responses, shedding light on its potential role in various diseases and therapeutic targets. The Mouse G-CSF ELISA Kit - High Sensitivity, developed by Assay Genie, offers exceptional sensitivity and specificity, ensuring reliable and reproducible results for advanced research applications. With stringent quality control measures and optimized performance, this kit delivers robust and user-friendly protocols, making it an excellent choice for investigating the role of G-CSF in mouse models and advancing scientific discoveries in immunology and hematopoiesis.
Product Name:
Mouse G-CSF ELISA Kit (High Sensitivity)
SKU:
AEES00608
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
8.38 pg/mL
Detection range:
15.62-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
