The Mouse GM-CSF (Granulocyte-Macrophage Colony-Stimulating Factor) ELISA Kit - High Sensitivity is a specialized assay designed to quantitatively analyze levels of GM-CSF in various biological samples from mouse models. GM-CSF is a crucial cytokine that plays a key role in regulating granulocyte and macrophage production, immune responses, and hematopoietic processes. By providing accurate and high-sensitivity measurements of GM-CSF levels, this ELISA kit enables researchers to delve into the intricate mechanisms underlying immune modulation, myeloid cell development, and inflammatory responses. This ELISA kit is tailored to detect GM-CSF with exceptional sensitivity, allowing researchers to explore the dynamic interactions between GM-CSF and immune cell regulation. By investigating GM-CSF levels in mouse samples, researchers can gain insights into its involvement in hematopoiesis, immune system function, and potential implications in various diseases and therapeutic interventions. Manufactured by Assay Genie, the Mouse GM-CSF ELISA Kit - High Sensitivity ensures reliable and reproducible results with stringent quality control standards. With its exceptional sensitivity and specificity, this kit offers robust performance and ease of use, making it an indispensable tool for research aimed at elucidating the role of GM-CSF in immune responses, myeloid cell regulation, and disease pathogenesis.
Product Name:
Mouse GM-CSF ELISA Kit (High Sensitivity)
SKU:
AEES00609
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
4.67 pg/mL
Detection range:
7.81-500 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
