Anti-Nck Antibody (CAB9129)

SKU:
CAB9129
€129 - €349

Description

Technical Manual

Anti-Nck Antibody

Product Name:Anti-Nck Antibody
Product SKU:CAB9129
Size:20uL, 50 uL
Host Species:Rabbit
Purification Method:Affinity purification
Isotype:IgG
Background:

The protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. Alternatively spliced transcript variants encoding different isoforms have been found. [provided by RefSeq, Jun 2010]

Immunogen:A synthesized peptide derived from human Nck
Sequence:

Email for sequence

Gene ID:4690
Swiss Prot:P16333
Synonyms:NCK; NCKalpha; nck-1
Calculated MW:47kDa
Observed MW:47KDa
Reactivity:Human, Mouse, Rat
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application Key:Western blotting Immunohistochemistry Immunofluorescence
Positive Samples:HeLa, 293T, Mouse lung, Mouse brain, Rat brain
Cellular Location:

Product Images

Anti-Nck Antibody (CAB9129)

Western blot - Nck Rabbit mAb (CAB9129)

Uniprot Information

UniProt Protein Function:NCK1: adapter protein containing Src homology 2 and 3 (SH2 and SH3) domains. Transduces signals from tyrosine-phosphorylated growth factor receptors to their cellular substrates.
UniProt Protein Details:

Protein type:Adaptor/scaffold; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 3q21

Cellular Component: endoplasmic reticulum; cytoplasm; plasma membrane; ribosome; protein phosphatase type 1 complex; intercellular junction; vesicle membrane; cytosol; nucleus

Molecular Function:protein binding, bridging; protein domain specific binding; protein binding; receptor signaling complex scaffold activity; protein kinase inhibitor activity; SH3/SH2 adaptor activity; cytoskeletal adaptor activity; receptor tyrosine kinase binding; receptor binding

Biological Process: lamellipodium biogenesis; axon guidance; positive regulation of translation in response to stress; T cell activation; negative regulation of peptidyl-serine phosphorylation; signal complex assembly; actin filament organization; response to other organism; T cell receptor signaling pathway; substrate-bound cell migration, cell extension; positive regulation of actin filament polymerization; negative regulation of protein kinase activity; innate immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of T cell proliferation; vascular endothelial growth factor receptor signaling pathway

NCBI Summary:The protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. Alternatively spliced transcript variants encoding different isoforms have been found. [provided by RefSeq, Jun 2010]
UniProt Code:P16333
NCBI GenInfo Identifier:127962
NCBI Gene ID:4690
NCBI Accession:P16333.1
UniProt Secondary Accession:P16333,B7Z751, D3DNE3,
UniProt Related Accession:P16333
Molecular Weight:377
NCBI Full Name:Cytoplasmic protein NCK1
NCBI Synonym Full Names:NCK adaptor protein 1
NCBI Official Symbol:NCK1
NCBI Official Synonym Symbols:NCK; nck-1; NCKalpha
NCBI Protein Information:cytoplasmic protein NCK1; NCK tyrosine kinase; melanoma NCK protein; SH2/SH3 adaptor protein NCK-alpha; non-catalytic region of tyrosine kinase
UniProt Protein Name:Cytoplasmic protein NCK1
UniProt Synonym Protein Names:NCK adaptor protein 1; Nck-1; SH2/SH3 adaptor protein NCK-alpha
Protein Family:Nck-associated protein
UniProt Gene Name:NCK1
UniProt Entry Name:NCK1_HUMAN
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Additional Information

Product type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Monoclonal Antibody
Research Area:
Signal Transduction
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