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Western blot analysis of extracts of A-549 cells, using NDUFC2 antibody (CAB3312). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Key Features

NDUFC2 Polyclonal Antibody (CAB3312)

Datasheet MSDS
SKU CAB3312
Product Type Antibody
Antibody Type Polyclonal Antibody
Reactivity Human, Mouse, Rat
Host Species Rabbit
Synonyms HLC-1, B14.5b, MC1DN36, NADHDH2, CI-B14.5b, NDUFC2
€96 - €370

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Description

system_update_altDatasheetsystem_update_altMSDS

NDUFC2 Polyclonal Antibody (CAB3312)

The NDUFC2 Polyclonal Antibody (CAB3312) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, boasts high specificity and sensitivity for human samples, making it an excellent choice for Western blot applications. By targeting the NDUFC2 protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it an invaluable resource for studies in bioenergetics and mitochondrial function.

This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:NDUFC2 Polyclonal Antibody
SKU:CAB3312
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:MIAR RNPE PLRF LPDE ARSL PPPK LTDP RLLY IGFL GYCS GLID NLIR RRPI ATAG LHRQ LLYI TAFF FAGY YLVK REDY LYAV RDRE MFGY MKLH PEDF
Tested Applications:WB ELISA
Recommended Dilution:
WB1:500 - 1:2000
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:HLC-1, B14.5b, MC1DN36, NADHDH2, CI-B14.5b, NDUFC2
Positive Sample:A-549
Cellular Localization:Matrix Side, Mitochondrion Inner Membrane, Single-Pass Membrane Protein.
Calculated MW:14kDa
Observed MW:14kDa

Involved in mitochondrial respiratory chain complex I assembly. Located in mitochondrion. Part of mitochondrial respiratory chain complex I. Implicated in nuclear type mitochondrial complex I deficiency.

Purification MethodAffinity purification
Gene ID4718
RRIDAB_2765042
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from A-549 cells, using NDUFC2 Rabbit pAb (CAB3312). Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
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