Anti-NEK8 Antibody (CAB0984)

Antibody Name:	Anti-NEK8 Antibody
Antibody SKU:	CAB0984
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human NEK8 (NP_835464.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, Jurkat, K-562, Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human NEK8 (NP_835464.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MEKY ERIR VVGR GAFG IVHL CLRK ADQK LVII KQIP VEQM TKEE RQAA QNEC QVLK LLNH PNVI EYYE NFLE DKAL MIAM EYAP GGTL AEFI QKRC NSLL EEET ILHF FVQI LLAL HHVH THLI LHRD LKTQ NILL DKHR MVVK IGDF GISK ILSS KSKA YTVV GTPC YISP ELCE GKPY KNVS SATH SACV LYEL ASLK RAFE AANL PALV LKIM SGTF APIS DRYS PELR QLVL SLLS LEPA QRPP LSHI MAQP LCIR ALLN LHTD VGSV RMRR AEKS VAPS NTGS RTTS VRCR GIPR
Gene ID:	284086
Uniprot:	Q86SG6
Cellular Location:	Cell projection, Cytoplasm, cilium, cytoskeleton
Calculated MW:	74kDa
Observed MW:	80kDa

Synonyms:

NEK8, JCK, NEK12A, NPHP9, RHPD2

Background:

This gene encodes a member of the serine/threionine protein kinase family related to NIMA (never in mitosis, gene A) of Aspergillus nidulans. The encoded protein may play a role in cell cycle progression from G2 to M phase. Mutations in the related mouse gene are associated with a disease phenotype that closely parallels the juvenile autosomal recessive form of polycystic kidney disease in humans.

UniProt Protein Function:	NEK8: a protein kinase related to the NIMA (never in mitosis, gene A) serine/threonine kinase of Aspergillus nidulans. May play a role in mitotic regulation.Required for renal tubular integrity.
UniProt Protein Details:	Protein type:EC 2.7.11.1; Protein kinase, tyrosine (non-receptor); Kinase, protein; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); Other group; NEK family Chromosomal Location of Human Ortholog: 17q11.1 Cellular Component: cytoskeleton; cytoplasm Molecular Function:protein serine/threonine kinase activity; protein binding; metal ion binding; ATP binding Biological Process: organ morphogenesis; mitotic cell cycle; protein amino acid phosphorylation; microtubule-based process Disease: Renal-hepatic-pancreatic Dysplasia 2; Nephronophthisis 9
NCBI Summary:	This gene encodes a member of the serine/threionine protein kinase family related to NIMA (never in mitosis, gene A) of Aspergillus nidulans. The encoded protein may play a role in cell cycle progression from G2 to M phase. Mutations in the related mouse gene are associated with a disease phenotype that closely parallels the juvenile autosomal recessive form of polycystic kidney disease in humans. [provided by RefSeq, Jul 2008]
UniProt Code:	Q86SG6
NCBI GenInfo Identifier:	34098463
NCBI Gene ID:	284086
NCBI Accession:	Q86SG6.1
UniProt Secondary Accession:	Q86SG6,Q14CL7, Q2M1S6, Q8NDH1, A6NIC5,
UniProt Related Accession:	Q86SG6
Molecular Weight:	Calculated MW: 74kDaObserved MW: 80kDa
NCBI Full Name:	Serine/threonine-protein kinase Nek8
NCBI Synonym Full Names:	NIMA-related kinase 8
NCBI Official Symbol:	NEK8
NCBI Official Synonym Symbols:	JCK; NPHP9; RHPD2; NEK12A
NCBI Protein Information:	serine/threonine-protein kinase Nek8; nephrocystin 9; NIMA-family kinase NEK8; NIMA-related kinase 12a; nimA-related protein kinase 8; nima-related protein kinase 12a; never in mitosis A-related kinase 8; serine/thrionine-protein kinase NEK8; NIMA (never in mitosis gene a)- related kinase 8
UniProt Protein Name:	Serine/threonine-protein kinase Nek8
UniProt Synonym Protein Names:	Never in mitosis A-related kinase 8; NimA-related protein kinase 8; Nima-related protein kinase 12a
Protein Family:	Serine/threonine-protein kinase
UniProt Gene Name:	NEK8
UniProt Entry Name:	NEK8_HUMAN

	Western blot analysis of extracts of various cell lines, using NEK8 antibody (CAB0984) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunofluorescence analysis of HeLa cells using NEK8 antibody (CAB0984). Blue: DAPI for nuclear staining.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)