Description
NETosis Colorimetric Assay Kit (MAES0482)
The NETosis Colorimetric Assay Kit offers a convenient and reliable method for quantifying NETosis, a unique form of programmed cell death in neutrophils. Upon activation by pathogens, inflammatory mediators, or chemical stimuli, neutrophils release neutrophil extracellular traps (NETs)—web-like structures composed of chromatin and granule proteins—that help trap and neutralize invading microbes. Unlike apoptosis or necrosis, NETosis involves a distinct biochemical pathway regulated by enzymes such as neutrophil elastase (NE), myeloperoxidase (MPO), and PAD4.
This kit specifically measures the enzymatic activity of NE as a marker of NETosis using a colorimetric detection method. It is optimized for use with cell samples and requires only 20 μL of cell homogenate per assay. With a detection range of 0.31–18.33 U/L and high sensitivity (0.31 U/L), the assay provides robust and reproducible results within 7 hours and 20 minutes. Ideal for research in immunology, inflammation, and metabolic diseases, the kit is compatible with standard microplate readers (360–400 nm, optimal at 380 nm) and is suitable for high-throughput experimental workflows.
| Product Name: | NETosis Colorimetric Assay Kit |
| SKU: | MAES0482 |
| Size: | 96T |
| Detection Method: | Colorimetric Method |
| Sensitivity: | 0.31 U/L |
| Range: | 0.31–18.33 U/L |
| Sample Type: | Cell |
| Detection Instrument: | Microplate Reader (360–400 nm, optimum wavelength: 380 nm) |
| Research Area: | Metabolic Diseases |
| Other Reagents Required: | Basal Culture Medium, DMSO |
| Storage: | This product can be stored at -20°C for 12 months with shading light. |
| Precision: | Inter-assay CV: 4.8–7.4% Intra-assay CV: 2.1–3.8% |
| Sample Volume: | 20 μL (Cell Homogenate) |
| Assay Time: | 7 h 20 min |
NETosis is a distinct form of inflammatory cell death occurring in neutrophils. During this process, activated neutrophils release neutrophil extracellular traps (NETs), which are composed of decondensed chromatin and granule-derived proteins. The formation of NETs is closely associated with neutrophil death and is mechanistically different from apoptosis and necrosis. Various stimuli—such as pathogens (bacteria, fungi, viruses), activated platelets, chemokines (e.g., interleukins, G-CSF, TGF-β), synthetic compounds, and calcium ionophores—can trigger NETosis by promoting the expression and activity of key enzymes, including neutrophil elastase (NE), myeloperoxidase (MPO), and peptidylarginine deiminase 4 (PAD4). This assay evaluates NETosis by quantifying the enzymatic activity of NE, serving as a direct indicator of NET formation in neutrophils.
