Nitric Oxide Assay Kit - Colorimetric (MAES0050)
- SKU:
- MAES0050
- Product Type:
- Assay
- Detection Method:
- Colorimetric
- Instrument:
- Microplate Reader
- Sample Type:
- Serum, Plasma, Tissue, Saliva
- Sensitivity:
- 0.16 umol/L
- Range:
- 0.16-100 umol/L
- Assay Time:
- 55 min
- Shipping:
- Gel Pack
- Research Area:
- Oxidative Stress
- Reactive Oxygen Species
- Food Safety & Analysis
Description
| Product Name: | Nitric Oxide Assay Kit - Colorimetric |
| Product Code: | MAES0050 |
| Product Size: | 96 Assays |
| Sample Type: | Serum, plasma, tissue, saliva |
| Assay Time: | 55 min |
| Instrument: | Microplate reader |
| Sensitivity: | 0.16 µmol/L |
| Detection Range: | 0.16-100 µmol/L |
| Recovery Rate: | 102 |
| Inter CV: | 3.7 |
| Inter CV: | 2.4 |
The half-life of NO is extremely short, it exists in form of nitrate or nitrite produced by vascular endothelial cell, vascular smooth muscle cell, platelet, and macrophage and so on. The concentration of NO can be indirectly measured by detecting that of nitrate or nitrite. NO react with oxygen and water to generate nitrate or nitrite which can form a kind of pale red azo compound when meet with nitrate chromogenic reagent, the absorbance of the compound can be measured to calculate the concentration of NO indirectly.
- in vivo or in aqueous solution, and a reddish azo NO is easily oxidized to form NO2 compound is formed with the color developing agent, and the concentration of the azo compound is linearly related to the concentration of NO. The concentration of NO can be calculated indirectly by measuring the OD value at 550 nm.
| Shelf Life: | 6 Months |
| Storage: | 2-8°C |
| Shipping: | Gel Pack |
| Research Area: | Oxidative stress |
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