Nitric Oxide Assay Kit (Colorimetric) (BA0007)

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Nitiric Oxide Assay Kit - Information

Assay Genie's Nitric Oxide Assay Kit is designed to accurately measure NO production following reduction of nitrate to nitrite using improved Griess method. The procedure is simple and the time required for sample pretreatment and assay is reduced to as short as 30 min.


For quantitative determination of nitric oxide and evaluation of drug effects on its metabolism.

Nitric Oxide Assay Kit - Key Features

  • Sensitive and accurate. Detection range 0.6 - 200 in 96-well plate.
  • Rapid and reliable. Using an optimized VCl3 reagent, the time required for reduction of NO3- to NO2- is 10 min at 60°C.
  • Simple and high-throughput. The procedure involves mixing sample with three reagents, incubation for 10 min at 60°C and reading the optical density. Can be readily automated to measure thousands of samples per day.

Nitric Oxide Assay Kit - Data Sheet

Kit IncludesReagent A: 12 mL Reagent B: 500 uL Reagent C: 12 mL NaOH: 1 mL ZnSO4: 1 mL Standard: 1 mL
Kit RequiresPipetting devices, eppendorf tubes, eppendorf centrifuge, clear, flat bottomed 96 well plates or cuvettes, plate reader or spectrophotometer and heat block or hot water bath (optional).
Method of DetectionOD540nm
Detection Limit0.6
SamplesPlasma, serum, urine, tissue/cells and foods
Protocol Length40 min
Size100 tests
StorageStore the Standard at -20°C and all other reagents at 2-8°C.
Shelf Life6 months

More Details

Nitric oxide (NO) is a reactive radical that plays an important role in many key physiological functions. NO, an oxidation product of arginine by nitric oxide synthase, is involved in host defense and development, activation of regulatory proteins and direct covalent interaction with functional biomolecules. Simple, direct and automation-ready procedures for measuring NO are becoming popular in Research and Drug Discovery. Since NO is oxidized to nitrite and nitrate, it is common practice to quantitate total NO2-/NO3- as a measure for NO level.
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