Anti-NKX2-8 Antibody (PACO57892)

Antibody Name:	NKX2-8 Antibody (PACO57892)
Antibody SKU:	PACO57892
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, IHC
Recommended Dilutions:	ELISA:1:2000-1:10000, IHC:1:200-1:500
Species Reactivity:	Human
Immunogen:	Recombinant Human Homeobox protein Nkx-2.8 protein (1-160AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

IHC image of PACO57892 diluted at 1:300 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of PACO57892 diluted at 1:300 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Background:

double-stranded DNA binding, RNA polymerase II core promoter proximal region sequence-specific DNA binding, sequence-specific DNA binding, transcription factor activity, sequence-specific DNA binding, transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding, liver development, positive regulation of transcription from RNA polymerase II promoter, transcription from RNA polymerase II promoter, transcription, DNA-templated

Synonyms:

Homeobox protein Nkx-2.8 (Homeobox protein NK-2 homolog H), NKX2-8, NKX-2.8 NKX2G NKX2H

UniProt Protein Function:	NKX2-8: Belongs to the NK-2 homeobox family.Protein type: Cell development/differentiation; DNA-binding; Transcription factorChromosomal Location of Human Ortholog: 14q13.3Molecular Function: double-stranded DNA binding; sequence-specific DNA binding; transcription factor activityBiological Process: positive regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter; transcription, DNA-dependent
UniProt Protein Details:
NCBI Summary:	The protein encoded by this gene is a homeobox-containing developmental regulator associated with liver development. The encoded protein binds to the alpha-fetoprotein (AFP) gene promoter and increases the expression of AFP. This gene is overexpressed in some lung cancers and is linked to poor patient survival, possibly due to its resistance to cisplatin. This gene is aberrantly methylated in pancreatic cancer, deleted in squamous cell lung carcinomas, and acts as a tumor suppressor in esophageal cancer. Mutations in this gene may also be a cause of neural tube defects. [provided by RefSeq, Dec 2015]
UniProt Code:	O15522
NCBI GenInfo Identifier:	206729883
NCBI Gene ID:	26257
NCBI Accession:	O15522.2
UniProt Secondary Accession:	O15522,Q8IUT7
UniProt Related Accession:	O15522
Molecular Weight:	25,866 Da
NCBI Full Name:	Homeobox protein Nkx-2.8
NCBI Synonym Full Names:	NK2 homeobox 8
NCBI Official Symbol:	NKX2-8
NCBI Official Synonym Symbols:	NKX2H; NKX2.8; Nkx2-9
NCBI Protein Information:	homeobox protein Nkx-2.8
UniProt Protein Name:	Homeobox protein Nkx-2.8
UniProt Synonym Protein Names:	Homeobox protein NK-2 homolog H
Protein Family:	Homeobox protein
UniProt Gene Name:	NKX2-8
UniProt Entry Name:	NKX28_HUMAN

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)