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Cell Biology Antibodies 17 Anti-NONO / p54nrb Antibody CAB3800
Key Features

NONO/p54nrb Rabbit Monoclonal Antibody (CAB3800)

Datasheet MSDS
SKU CAB3800
Product Type Antibody
Antibody Type Monoclonal Antibody
Reactivity Human, Mouse, Rat
Host Species Rabbit
Synonyms P54, NMT55, NRB54, MRXS34, P54NRB, PPP1R114, NONO/p54nrb
Research Use Cell Biology
€131 - €457

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Description

system_update_altDatasheetsystem_update_altMSDS

NONO/p54nrb Monoclonal Antibody (CAB3800)

The NONO/p54nrb Monoclonal Antibody (CAB3800) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is validated for use in various applications including Western blotting and immunofluorescence, enabling precise detection and analysis of Nono/P54nrb expression in different cell types.Nono/P54nrb is a multifunctional protein that plays essential roles in RNA splicing, transcriptional regulation, and DNA repair processes. Its involvement in these critical cellular functions makes it a valuable target for studies in molecular biology, cancer biology, and genetic disorders.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:NONO/p54nrb Monoclonal Antibody
SKU:CAB3800
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Clone Number:ARC0836
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:GTFP DARE QEIR MGQM AMGG AMGI NNRG AMPP APVP AGTP APPG PATM MPDG TLGL TPPT TERF GQAA TMEG IGAI GGTP PAFN RAAP GAEF APNK RRRY
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:1000 - 1:4000
IHC-P1:500 - 1:2000
IF/ICC1:100 - 1:1000
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:P54, NMT55, NRB54, MRXS34, P54NRB, PPP1R114, NONO/p54nrb
Positive Sample:MOLT-4, NIH/3T3, PC-12
Cellular Localization:Nucleus, Nucleus Speckle, Nucleolus.
Calculated MW:54kDa
Observed MW:60kDa

This gene encodes an RNA-binding protein which plays various roles in the nucleus, including transcriptional regulation and RNA splicing. A rearrangement between this gene and the transcription factor E3 gene has been observed in papillary renal cell carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes exist on Chromosomes 2 and 16.

Purification MethodAffinity purification
Gene ID4841
RRIDAB_2863142
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using NONO/p54nrb Rabbit mAb (CAB3800) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3min.
Product ImageWestern blot analysis of lysates from Rat liver, using NONO/p54nrb Rabbit mAb (CAB3800) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3min.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human tonsil tissue using NONO/p54nrb Rabbit mAb (CAB3800) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Mouse lung tissue using NONO/p54nrb Rabbit mAb (CAB3800) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Rat brain tissue using NONO/p54nrb Rabbit mAb (CAB3800) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Rat testis tissue using NONO/p54nrb Rabbit mAb (CAB3800) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Product ImageImmunofluorescence analysis of C6 cells using NONO/p54nrb Rabbit mAb (CAB3800) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Product ImageImmunofluorescence analysis of U-2 OS cells using NONO/p54nrb Rabbit mAb (CAB3800) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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