Anti-NOXO1 Antibody (CAB10477)

SKU:
CAB10477
€99 - €249

Description

Technical Manual

Anti-NOXO1 Antibody

Product Name:Anti-NOXO1 Antibody
Product SKU:CAB10477
Size:20uL, 50 uL
Host Species:Rabbit
Purification Method:Affinity purification
Isotype:IgG
Background:

This gene encodes an NADPH oxidase (NOX) organizer, which positively regulates NOX1 and NOX3. The protein contains a PX domain and two SH3 domains. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 121-370 of human NOXO1 (NP_653204.1).
Sequence:

QPLDLEPALPPGSRVILPTPEEQPLSRAAGRLSIHSLEAQSLRCLQPFCTQDTRDRPFQAQAQESLDVLLRHPSGWWLVENEDRQTAWFPAPYLEEAAPGQGREGGPSLGSSGPQFCASRAYESSRADELSVPAGARVRVLETSDRGWWLCRYGDRAGLLPAVLLRPEGLGALLSGTGFRGGDDPAGEARGFPEPSQATAPPPTVPTRPSPGAIQSRCCTVTRRALERRPRRQGRPRGCVDSVPHPTTEQ

Gene ID:124056
Swiss Prot:Q8NFA2
Synonyms:NOXO1; P41NOX; P41NOXA; P41NOXB; P41NOXC; SH3PXD5; SNX28
Calculated MW:40kDa/41kDa
Observed MW:41kDa
Reactivity:Mouse
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting
Positive Samples:Mouse small intestine, Mouse stomach
Cellular Location:Cell membrane, Cytoplasmic side, Peripheral membrane protein

Product Images

Anti-NOXO1 Antibody (CAB10477)

Western blot analysis of extracts of various cell lines, using NOXO1 antibody (CAB10477) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.

Uniprot Information

UniProt Protein Function:NOXO1: Constitutively potentiates the superoxide-generating activity of NOX1 and NOX3 and is required for the biogenesis of otoconia/otolith, which are crystalline structures of the inner ear involved in the perception of gravity. Isoform 3 is more potent than isoform 1 in activating NOX3. Together with NOXA1, may also substitute to NCF1/p47phox and NCF2/p67phox in supporting the phagocyte NOX2/gp91phox superoxide-generating activity. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Adaptor/scaffold

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: NADPH oxidase complex; plasma membrane

Molecular Function:enzyme binding; identical protein binding; phospholipid binding; protein binding; superoxide-generating NADPH oxidase activator activity

Biological Process: extracellular matrix disassembly; regulation of hydrogen peroxide metabolic process

NCBI Summary:This gene encodes an NADPH oxidase (NOX) organizer, which positively regulates NOX1 and NOX3. The protein contains a PX domain and two SH3 domains. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jun 2012]
UniProt Code:Q8NFA2
NCBI GenInfo Identifier:74751269
NCBI Gene ID:124056
NCBI Accession:Q8NFA2.1
UniProt Secondary Accession:Q8NFA2,Q86YM1, Q8NFA3, Q96B73,
UniProt Related Accession:Q8NFA2
Molecular Weight:40,668 Da
NCBI Full Name:NADPH oxidase organizer 1
NCBI Synonym Full Names:NADPH oxidase organizer 1
NCBI Official Symbol:NOXO1
NCBI Official Synonym Symbols:SNX28; P41NOX; P41NOXA; P41NOXB; P41NOXC; SH3PXD5
NCBI Protein Information:NADPH oxidase organizer 1
UniProt Protein Name:NADPH oxidase organizer 1
UniProt Synonym Protein Names:NADPH oxidase regulatory protein; Nox organizer 1; Nox-organizing protein 1; SH3 and PX domain-containing protein 5
Protein Family:NADPH oxidase organizer
UniProt Gene Name:NOXO1
UniProt Entry Name:NOXO1_HUMAN
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Additional Information

Product type:
Antibody
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
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