The ENO2 Monoclonal Antibody (CAB19091) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ENO2 Monoclonal Antibody
SKU:
CAB19091
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC52246
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PIF/ICCELISA
Recommended Dilution:
WB
1:1000 - 1:6000
IHC-P
1:500 - 1:2000
IF/ICC
1:100 - 1:800
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
NSE, HEL-S-279, ENO2
Positive Sample:
SH-SY5Y, Mouse brain, Rat brain
Cellular Localization:
Cell Membrane, Cytoplasm.
Calculated MW:
47kDa
Observed MW:
47kDa
This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates.
Purification Method
Affinity purification
Gene ID
2026
RRID
AB_2862583
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using ENO2 Rabbit mAb (CAB19091)at 1:10000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Negative control (NC): U-937 Exposure time: 15s.
Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using ENO2 Rabbit mAb (CAB19091) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using ENO2 Rabbit mAb (CAB19091) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Confocal imaging of Neuro-2a cells using ENO2 Rabbit mAb (CAB19091,dilution 1:100)(Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012,dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
