Anti-NUDT1 Antibody (CAB13330)

Antibody Name:	Anti-NUDT1 Antibody [KO Validated]
Antibody SKU:	CAB13330
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IP
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-179 of human NUDT1 (NP_945192.1).

Application:	WB IHC IP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IP 1:20 - 1:50
Reactivity:	Human
Positive Samples:	SW480, K562, 22RV1, THP-1, HepG2, SKOV3, 293T

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-179 of human NUDT1 (NP_945192.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSGI SPQQ MGEP EGSW SGKN PGTM GASR LYTL VLVL QPQR VLLG MKKR GFGA GRWN GFGG KVQE GETI EDGA RREL QEES GLTV DALH KVGQ IVFE FVGE PELM DVHV FCTD SIQG TPVE SDEM RPCW FQLD QIPF KDMW PDDS YWFP LLLQ KKKF HGYF KFQG QDTI LDYT LREV DTV
Gene ID:	4521
Uniprot:	P36639
Cellular Location:	Cytoplasm, Mitochondrion matrix, Nucleus
Calculated MW:	17kDa/19kDa/20kDa/22kDa
Observed MW:	18kDa

Synonyms:

NUDT1, MTH1

Background:

Misincorporation of oxidized nucleoside triphosphates into DNA/RNA during replication and transcription can cause mutations that may result in carcinogenesis or neurodegeneration. The protein encoded by this gene is an enzyme that hydrolyzes oxidized purine nucleoside triphosphates, such as 8-oxo-dGTP, 8-oxo-dATP, 2-hydroxy-dATP, and 2-hydroxy rATP, to monophosphates, thereby preventing misincorporation. The encoded protein is localized mainly in the cytoplasm, with some in the mitochondria, suggesting that it is involved in the sanitization of nucleotide pools both for nuclear and mitochondrial genomes. Several alternatively spliced transcript variants, some of which encode distinct isoforms, have been identified. Additional variants have been observed, but their full-length natures have not been determined. A single-nucleotide polymorphism that results in the production of an additional, longer isoform (p26) has been described.

UniProt Protein Function:	NUDT1: Antimutagenic. Acts as a sanitizing enzyme for oxidized nucleotide pools, thus suppressing cell dysfunction and death induced by oxidative stress. Hydrolyzes 8-oxo-dGTP, 8-oxo-dATP and 2-OH-dATP, thus preventing misincorporation of oxidized purine nucleoside triphosphates into DNA and subsequently preventing A:T to C:G and G:C to T:A transversions. Able to hydrolyze also the corresponding ribonucleotides, 2-OH-ATP, 8-oxo-GTP and 8-oxo-ATP. Belongs to the Nudix hydrolase family. 4 isoforms of the human protein are produced by alternative initiation.
UniProt Protein Details:	Protein type:EC 3.6.1.55; Hydrolase; EC 3.6.1.56 Chromosomal Location of Human Ortholog: 7p22 Cellular Component: mitochondrion; mitochondrial matrix; cytoplasm; nucleus; cytosol Molecular Function:GTPase activity; snoRNA binding; m7G(5')pppN diphosphatase activity; metal ion binding; ATP diphosphatase activity; 8-oxo-7,8-dihydroguanine triphosphatase activity Biological Process: DNA protection; dGTP catabolic process; nucleobase, nucleoside and nucleotide metabolic process; dATP catabolic process; purine nucleotide catabolic process; response to oxidative stress; DNA repair
NCBI Summary:	Misincorporation of oxidized nucleoside triphosphates into DNA/RNA during replication and transcription can cause mutations that may result in carcinogenesis or neurodegeneration. The protein encoded by this gene is an enzyme that hydrolyzes oxidized purine nucleoside triphosphates, such as 8-oxo-dGTP, 8-oxo-dATP, 2-hydroxy-dATP, and 2-hydroxy rATP, to monophosphates, thereby preventing misincorporation. The encoded protein is localized mainly in the cytoplasm, with some in the mitochondria, suggesting that it is involved in the sanitization of nucleotide pools both for nuclear and mitochondrial genomes. Several alternatively spliced transcript variants, some of which encode distinct isoforms, have been identified. Additional variants have been observed, but their full-length natures have not been determined. A single-nucleotide polymorphism that results in the production of an additional, longer isoform (p26) has been described. [provided by RefSeq, Jul 2008]
UniProt Code:	P36639
NCBI GenInfo Identifier:	254763430
NCBI Gene ID:	4521
NCBI Accession:	P36639.3
UniProt Secondary Accession:	P36639,Q6LES7, Q6P0Y6, Q7Z7N6, Q8IV95, Q9UBM0, Q9UBM9 A4D205,
UniProt Related Accession:	P36639
Molecular Weight:	197
NCBI Full Name:	7,8-dihydro-8-oxoguanine triphosphatase
NCBI Synonym Full Names:	nudix (nucleoside diphosphate linked moiety X)-type motif 1
NCBI Official Symbol:	NUDT1
NCBI Official Synonym Symbols:	MTH1
NCBI Protein Information:	7,8-dihydro-8-oxoguanine triphosphatase; 8-oxo-dGTPase; nudix motif 1; mutT human homolog 1; 2-hydroxy-dATP diphosphatase; 8-oxo-7,8-dihydroguanosine triphosphatase; 8-oxo-7,8-dihydrodeoxyguanosine triphosphatase; nucleoside diphosphate-linked moiety X motif 1; nucleoside diphosphate-linked moiety X-type motif 1
UniProt Protein Name:	7,8-dihydro-8-oxoguanine triphosphatase
UniProt Synonym Protein Names:	2-hydroxy-dATP diphosphatase (EC:3.6.1.56); 8-oxo-dGTPase; Nucleoside diphosphate-linked moiety X motif 1; Nudix motif 1
Protein Family:	Nudix hydrolase
UniProt Gene Name:	NUDT1
UniProt Entry Name:	8ODP_HUMAN

	Western blot analysis of extracts of various cell lines, using NUDT1 antibody (CAB13330) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Western blot analysis of extracts from normal (control) and NUDT1 knockout (KO) 293T cells, using NUDT1 antibody (CAB13330) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded human gastric cancer using NUDT1 antibody (CAB13330) at dilution of 1:100 (40x lens).
	Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug NUDT1 antibody (CAB13330). Western blot was performed from the immunoprecipitate using NUDT1 antibody (CAB13330) at a dilition of 1:1000.

Antibodies	ELISA Kits
Anti-NUDT1 Antibody (CAB5474)	Human MTH1 / NUDT1 ELISA Kit
Anti-MTH1 Antibody (CAB3938)	Human 7,8-dihydro-8-oxoguanine triphosphatase (NUDT1) ELISA Kit
NUDT1 Antibody (PACO10954)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)