Anti-NUP62 Antibody (CAB2499)

Antibody Name:	Anti-NUP62 Antibody
Antibody SKU:	CAB2499
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 403-522 of human NUP62 (NP_036478.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:10 - 1:100
Reactivity:	Human
Positive Samples:	A-549, HeLa, HT-29, Raji, BT-474, U-937

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 403-522 of human NUP62 (NP_036478.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LEDL LSPL EELV KEQS GTIY LQHA DEER EKTY KLAE NIDA QLKR MAQD LKDI IEHL NTSG APAD TSDP LQQI CKIL NAHM DSLQ WIDQ NSAL LQRK VEEV TKVC EGRR KEQE RSFR ITFD
Gene ID:	23636
Uniprot:	P37198
Cellular Location:	Cytoplasm, Nucleus, cytoskeleton, nuclear pore complex, spindle pole
Calculated MW:	53kDa
Observed MW:	62kDa

Synonyms:

NUP62, IBSN, SNDI, p62

Background:

The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins and is localized to the nuclear pore central plug. This protein associates with the importin alpha/beta complex which is involved in the import of proteins containing nuclear localization signals. Multiple transcript variants of this gene encode a single protein isoform.

UniProt Protein Function:	NUP62: Essential component of the nuclear pore complex. The N- terminal is probably involved in nucleocytoplasmic transport. The C-terminal is probably involved in protein-protein interaction via coiled-coil formation and may function in anchorage of p62 to the pore complex. Defects in NUP62 are the cause of infantile striatonigral degeneration (SNDI); also known as infantile bilateral striatal necrosis (IBSN) or familial striatal degeneration. SNDI is a neurological disorder characterized by symmetrical degeneration of the caudate nucleus, putamen and occasionally the globus pallidus, with little involvement of the rest of the brain. The clinical features include developmental regression, choreoathetosis, dystonia, spasticity, dysphagia, failure to thrive, nystagmus, optic atrophy and mental retardation. Belongs to the nucleoporin NSP1/NUP62 family.
UniProt Protein Details:	Protein type:Nucleoporin; Adaptor/scaffold Chromosomal Location of Human Ortholog: 19q13.33 Cellular Component: pore complex; spindle pole; nucleocytoplasmic shuttling complex; nuclear membrane; intracellular membrane-bound organelle; cytoplasm; nuclear envelope; nuclear pore; ribonucleoprotein complex Molecular Function:ubiquitin binding; protein binding; receptor signaling complex scaffold activity; structural constituent of nuclear pore; PTB domain binding; chromatin binding; SH2 domain binding; thyroid hormone receptor binding; nucleocytoplasmic transporter activity Biological Process: cell death; negative regulation of epidermal growth factor receptor signaling pathway; negative regulation of MAP kinase activity; mRNA transport; viral reproduction; hormone-mediated signaling; positive regulation of transcription, DNA-dependent; mitotic nuclear envelope disassembly; regulation of signal transduction; nucleocytoplasmic transport; pathogenesis; viral infectious cycle; glucose transport; negative regulation of cell proliferation; protein transport; cell surface receptor linked signal transduction; positive regulation of epidermal growth factor receptor signaling pathway; negative regulation of programmed cell death; negative regulation of Ras protein signal transduction; viral transcription; transmembrane transport; regulation of Ras protein signal transduction; positive regulation of I-kappaB kinase/NF-kappaB cascade; transcription, DNA-dependent; cytokine and chemokine mediated signaling pathway; cell aging; hexose transport; carbohydrate metabolic process; gene expression; mitotic cell cycle; negative regulation of apoptosis Disease: Striatonigral Degeneration, Infantile
NCBI Summary:	The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins and is localized to the nuclear pore central plug. This protein associates with the importin alpha/beta complex which is involved in the import of proteins containing nuclear localization signals. Multiple transcript variants of this gene encode a single protein isoform. [provided by RefSeq, Jul 2008]
UniProt Code:	P37198
NCBI GenInfo Identifier:	134047855
NCBI Gene ID:	23636
NCBI Accession:	P37198.3
UniProt Related Accession:	P37198
Molecular Weight:
NCBI Full Name:	Nuclear pore glycoprotein p62
NCBI Synonym Full Names:	nucleoporin 62
NCBI Official Symbol:	NUP62
NCBI Official Synonym Symbols:	p62; IBSN; SNDI
NCBI Protein Information:	nuclear pore glycoprotein p62
UniProt Protein Name:	Nuclear pore glycoprotein p62
UniProt Synonym Protein Names:	62 kDa nucleoporin; Nucleoporin Nup62
Protein Family:	Nuclear pore glycoprotein
UniProt Gene Name:	NUP62
UniProt Entry Name:	NUP62_HUMAN

	Western blot analysis of extracts of various cell lines, using NUP62 antibody (CAB2499) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020).
	Immunohistochemistry of paraffin-embedded human lung cancer using NUP62 antibody (CAB2499) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using NUP62 antibody (CAB2499). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
NUP62 Antibody (PACO10973)	Human Nucleoporin p62 / NUP62 ELISA Kit
	Human NUP62 (Nucleoporin 62kDa) CLIA Kit (HUES01245)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)