The NXN Antibody (CAB17206) is a high-quality antibody developed for reliable detection and analysis of target proteins. Raised in rabbits, this antibody is highly specific for human samples and has been validated for use in Western blot applications. By binding to the NXN protein, this antibody enables precise detection and analysis in a variety of cell types, making it ideal for investigations in fields such as cell biology, cancer research, and oxidative stress mechanisms.NXN, known for its role in mediating redox signaling pathways and protection against oxidative damage, is a key player in cellular defense mechanisms. Its involvement in processes such as DNA repair and apoptosis highlights its importance in maintaining cellular homeostasis.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
NXN Antibody
SKU:
CAB17206
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
NRX, RRS2, TRG-4, NXN
Positive Sample:
Rat testis
Cellular Localization:
Cytosol, Nucleus.
Calculated MW:
48kDa
Observed MW:
48kDa
This gene encodes a member of the thioredoxin superfamily, a group of small, multifunctional redox-active proteins. Members of this family are characterized by a conserved active motif called the thioredoxin fold that catalyzes disulfide bond formation and isomerization. The encoded protein acts a redox-dependent regulator of the Wnt signaling pathway and is involved in cell growth and differentiation.
Purification Method
Affinity purification
Gene ID
64359
RRID
AB_2770704
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Rat testis, using NXN Rabbit pAb (CAB17206) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunofluorescence analysis of C6 cells using NXN Rabbit pAb (CAB17206) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of L929 cells using NXN Rabbit pAb (CAB17206) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
