Anti-OPRK1 Antibody (CAB14035)

Antibody Name:	Anti-OPRK1 Antibody
Antibody SKU:	CAB14035
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human OPRK1 (NP_000903.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse brain, Rat brain

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human OPRK1 (NP_000903.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	GSTS HSTA ALSS YYFC IALG YTNS SLNP ILYA FLDE NFKR CFRD FCFP LKMR MERQ STSR VRNT VQDP AYLR DIDG MNKP V
Gene ID:	4986
Uniprot:	P41145
Cellular Location:	Cell membrane, Multi-pass membrane protein
Calculated MW:	33kDa/42kDa
Observed MW:	40kDa

Synonyms:

OPRK1, K-OR-1, KOR, KOR-1, OPRK

Background:

This gene encodes an opioid receptor, which is a member of the 7 transmembrane-spanning G protein-coupled receptor family. It functions as a receptor for endogenous ligands, as well as a receptor for various synthetic opioids. Ligand binding results in inhibition of adenylate cyclase activity and neurotransmitter release. This opioid receptor plays a role in the perception of pain and mediating the hypolocomotor, analgesic and aversive actions of synthetic opioids. Variations in this gene have also been associated with alcohol dependence and opiate addiction. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. A recent study provided evidence for translational readthrough in this gene and expression of an additional C-terminally extended isoform via the use of an alternative in-frame translation termination codon.

UniProt Protein Function:	KOR-1: kappa-type opioid receptor. Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Receptor for dynorphins. May play a role in arousal and regulation of autonomic and neuroendocrine functions.
UniProt Protein Details:	Protein type:Receptor, GPCR; Membrane protein, multi-pass; Membrane protein, integral; GPCR, family 1 Chromosomal Location of Human Ortholog: 8q11.2 Cellular Component: dendrite; integral to membrane; integral to plasma membrane; nerve terminal; neuron projection; perikaryon; plasma membrane; synapse Molecular Function:neuropeptide binding; opioid receptor activity; protein binding Biological Process: behavior; behavioral response to cocaine; defense response to virus; eating behavior; G-protein signaling, adenylate cyclase inhibiting pathway; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); immune response; locomotory behavior; maternal behavior; negative regulation of luteinizing hormone secretion; neuropeptide signaling pathway; opioid receptor, adenylate cyclase inhibiting pathway; positive regulation of dopamine secretion; positive regulation of locomotion; regulation of saliva secretion; regulation of sensory perception of pain; response to estrogen stimulus; response to ethanol; response to insulin stimulus; response to morphine; response to radiation; sensory perception; sensory perception of pain; sensory perception of temperature stimulus; synaptic transmission
NCBI Summary:	This gene encodes an opioid receptor, which is a member of the 7 transmembrane-spanning G protein-coupled receptor family. It functions as a receptor for endogenous ligands, as well as a receptor for various synthetic opioids. Ligand binding results in inhibition of adenylate cyclase activity and neurotransmitter release. This opioid receptor plays a role in the perception of pain and mediating the hypolocomotor, analgesic and aversive actions of synthetic opioids. Variations in this gene have also been associated with alcohol dependence and opiate addiction. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. A recent study provided evidence for translational readthrough in this gene and expression of an additional C-terminally extended isoform via the use of an alternative in-frame translation termination codon. [provided by RefSeq, Jan 2016]
UniProt Code:	P41145
NCBI GenInfo Identifier:	116242691
NCBI Gene ID:	4986
NCBI Accession:	P41145.2
UniProt Secondary Accession:	P41145,Q499G4, E5RHC9,
UniProt Related Accession:	P41145
Molecular Weight:	33,319 Da
NCBI Full Name:	Kappa-type opioid receptor
NCBI Synonym Full Names:	opioid receptor kappa 1
NCBI Official Symbol:	OPRK1
NCBI Official Synonym Symbols:	KOR; OPRK; KOR-1; K-OR-1
NCBI Protein Information:	kappa-type opioid receptor
UniProt Protein Name:	Kappa-type opioid receptor
Protein Family:	Kappa-type opioid receptor
UniProt Gene Name:	OPRK1
UniProt Entry Name:	OPRK_HUMAN

	Western blot analysis of extracts of various cell lines, using OPRK1 antibody (CAB14035) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunohistochemistry of paraffin-embedded rat testis using OPRK1 antibody (CAB14035) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human stomach using OPRK1 antibody (CAB14035) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse kidney using OPRK1 antibody (CAB14035) at dilution of 1:100 (40x lens).

Antibodies

Anti-OPRK1 Antibody (CAB8306)

OPRK1 Antibody (PACO03311)

OPRK1 Antibody (PACO04482)

Phospho-OPRK1 (S369) Antibody (PACO06161)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)