Anti-P2RY2 Antibody (CAB5779)

Antibody Name:	Anti-P2RY2 Antibody
Antibody SKU:	CAB5779
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 278-377 of human P2RY2 (NP_788086.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:10 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	Mouse liver, Mouse heart, Mouse skeletal muscle

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 278-377 of human P2RY2 (NP_788086.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	CHTL NAIN MAYK VTRP LASA NSCL DPVL YFLA GQRL VRFA RDAK PPTG PSPA TPAR RRLG LRRS DRTD MQRI EDVL GSSE DSRR TEST PAGS ENTK DIRL
Gene ID:	5029
Uniprot:	P41231
Cellular Location:	Cell membrane, Multi-pass membrane protein
Calculated MW:	42kDa
Observed MW:	50kDa

Synonyms:

P2RY2, HP2U, P2RU1, P2U, P2U1, P2UR, P2Y2, P2Y2R

Background:

The product of this gene belongs to the family of P2 receptors, which is activated by extracellular nucleotides and subdivided into P2X ligand-gated ion channels and P2Y G-protein coupled receptors. This family has several receptor subtypes with different pharmacological selectivity, which overlaps in some cases, for various adenosine and uridine nucleotides. This receptor, found on many cell types, is activated by ATP and UTP and is reported to be overexpressed on some cancer cell types. It is involved in many cellular functions, such as proliferation, apoptosis and inflammation. Three transcript variants encoding the same protein have been identified for this gene.

UniProt Protein Function:	P2Y2: a G-protein coupled receptor of the rhodopsin family. This family includes hormone, neurotransmitter and light receptors. Receptor for ATP and UTP coupled to G-proteins that activate a phosphatidylinositol-calcium second messenger system. The affinity range is UTP = ATP > ATP-gamma-S >> 2-methylthio-ATP = ADP. May participate in control of the cell cycle and proliferation.
UniProt Protein Details:	Protein type:Receptor, GPCR; Membrane protein, multi-pass; Receptor, misc.; GPCR, family 1; Membrane protein, integral Chromosomal Location of Human Ortholog: 11q13.5-q14.1 Cellular Component: integral to plasma membrane; plasma membrane Molecular Function:purinergic nucleotide receptor activity, G-protein coupled; receptor activity Biological Process: regulation of vasodilation; cellular ion homeostasis; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating)
NCBI Summary:	The product of this gene belongs to the family of P2 receptors, which is activated by extracellular nucleotides and subdivided into P2X ligand-gated ion channels and P2Y G-protein coupled receptors. This family has several receptor subtypes with different pharmacological selectivity, which overlaps in some cases, for various adenosine and uridine nucleotides. This receptor, found on many cell types, is activated by ATP and UTP and is reported to be overexpressed on some cancer cell types. It is involved in many cellular functions, such as proliferation, apoptosis and inflammation. Three transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Mar 2013]
UniProt Code:	P41231
NCBI GenInfo Identifier:	311033490
NCBI Gene ID:	5029
NCBI Accession:	P41231.4
UniProt Secondary Accession:	P41231,Q96EM8, B2R9W3,
UniProt Related Accession:	P41231
Molecular Weight:	377
NCBI Full Name:	P2Y purinoceptor 2
NCBI Synonym Full Names:	purinergic receptor P2Y, G-protein coupled, 2
NCBI Official Symbol:	P2RY2
NCBI Official Synonym Symbols:	P2U; HP2U; P2U1; P2UR; P2Y2; P2RU1; P2Y2R
NCBI Protein Information:	P2Y purinoceptor 2; ATP receptor; P2U receptor 1; purinoceptor P2Y2; P2U purinoceptor 1; P2U nucleotide receptor; purinergic receptor P2Y2
UniProt Protein Name:	P2Y purinoceptor 2
UniProt Synonym Protein Names:	ATP receptor; P2U purinoceptor 1; P2U1; P2U receptor 1; Purinergic receptor
Protein Family:	P2Y purinoceptor
UniProt Gene Name:	P2RY2
UniProt Entry Name:	P2RY2_HUMAN

	Western blot analysis of extracts of various cell lines, using P2RY2 antibody (CAB5779) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunofluorescence analysis of U2OS cells using P2RY2 antibody (CAB5779). Blue: DAPI for nuclear staining.

Antibodies

Anti-P2RY2 Antibody (CAB13923)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)