Western blot analysis of various lysates, using pan 14-3-3 Rabbit pAb (CAB23674) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The pan 14-3-3 Polyclonal Antibody (CAB23674) is a high-quality antibody developed for reliable detection and analysis of target proteins. The antibody, produced in rabbits, is highly reactive with human samples and is validated for use in various applications, including Western blotting and immunohistochemistry.The 14-3-3 proteins are highly conserved and interact with a wide range of target proteins, influencing different cellular processes. This antibody binds to the 14-3-3 protein, facilitating its detection and analysis in diverse cell types.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat, African green monkey samples.
Product Name:
pan 14-3-3 Polyclonal Antibody
SKU:
CAB23674
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat, African green monkey
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:
Email for sequence
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
YWHA, S1C5, HS1, 14-3-3, pan 14-3-3
Positive Sample:
HeLa, K-562, Jurkat, H-4-11-E, COS7, NIH/3T3
Calculated MW:
27kDa/28kDa
Observed MW:
27-29kDa
This gene encodes a cell cycle checkpoint protein. The encoded protein binds to translation and initiation factors and functions as a regulator of mitotic translation. In response to DNA damage this protein plays a role in preventing DNA errors during mitosis.This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease.
Purification Method
Affinity purification
Gene ID
10971 7529 7532 7531 7534 7533 2810
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using pan 14-3-3 Rabbit pAb (CAB23674) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 20s.
