The Pan Phospho-T Polyclonal Antibody (CABP1513) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific and reactive with phosphorylated threonine residues in proteins across various species, making it ideal for use in Western blot and immunohistochemistry applications.Phosphorylation of threonine residues is a key post-translational modification that regulates many cellular processes, including cell growth, proliferation, and differentiation. By targeting phosphorylated threonine residues in proteins, researchers can gain insights into the activation status of signaling pathways and the role of phosphorylation in disease processes.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Other (Wide Range Predicted) samples.
Product Name:
Pan Phospho-T Polyclonal Antibody
SKU:
CABP1513
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Other (Wide Range Predicted)
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Sample:
A-431 treated with Calyculin A, NIH/3T3 treated with Calyculin A
Observed MW:
10-250kDa/>10kDa
Purification Method
Affinity purification
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from A-431 cells using Pan Phospho-T Rabbit pAb (CABP1513) at 1:900 dilution. A-431 cells were treated with Calyculin A (50 nM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Western blot analysis of lysates from NIH/3T3 cells using Pan Phospho-T Rabbit pAb (CABP1513) at 1:900 dilution. NIH/3T3 cells were treated with Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
