Western blot analysis of A-431 or A 431 treat with Calyculin A, using Pan Phospho-Threonine Rabbit mAb antibody (CABP1422) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Key Features
Pan Phospho-Threonine Monoclonal Antibody (CABP1422)
Pan Phospho-Threonine Monoclonal Antibody (CABP1422)
The Pan Phospho-Threonine Monoclonal Antibody (CABP1422) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated through monoclonal antibody technology, exhibits high specificity and sensitivity for detecting phosphorylated threonine residues in a variety of samples, particularly in Western blot applications. Phosphorylation of threonine residues is a crucial post-translational modification that plays a key role in regulating protein function and cellular signaling pathways. By targeting phosphorylated threonine residues, researchers can gain insights into the mechanisms underlying various cellular processes, including cell growth, differentiation, and apoptosis.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat, Other (Wide Range Predicted) samples.
Product Name:
Pan Phospho-Threonine Monoclonal Antibody
SKU:
CABP1422
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat, Other (Wide Range Predicted)
Clone Number:
ARC61310
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIPELISA
Recommended Dilution:
WB
1:500 - 1:1000
IP
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Sample:
A-431 treated with Calyculin A , NIH/3T3 treated with Calyculin A ,
Observed MW:
10-250kDa
Purification Method
Affinity purification
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from A-431, using Pan Phospho-Threonine Rabbit mAb (CABP1422) at 1:1000 dilution. A 431 treated with Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 60s.
Western blot analysis of lysates from NIH 3T3 or NIH 3T3 treat with Calyculin A, using Pan Phospho-Threonine Rabbit mAb (CABP1422) at 1:1000 dilution. NIH/3T3 cells were treated with Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 60s.
