Western blot analysis of extracts from SH-SY5Y cells, using Phospho-Tau-S404 antibody (CABP1378) at 1:5000 dilution. SH-SY5Y cells were treated by Okadaic Acid (100 nM) at 37℃ for 1 hour and Calyculin A (100nM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The Phospho-Tau-S404 Monoclonal Antibody (CABP1378) is a high-quality antibody developed for reliable detection and analysis of target proteins. Tau protein plays a crucial role in the pathogenesis of Alzheimer's disease and other tauopathies, making it a key target for study in neurodegenerative research. This monoclonal antibody, produced through hybridoma technology, is highly specific and sensitive for detecting phosphorylated Tau at the S404 site. It is validated for use in immunohistochemistry, immunofluorescence, and other cellular assays, allowing for the visualization and quantification of phosphorylated Tau in various experimental settings.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Phospho-Tau-S404 Monoclonal Antibody
SKU:
CABP1378
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC58154
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:2000 - 1:6000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
This gene encodes the microtubule-associated protein tau (MAPT) whose transcript undergoes complex, regulated alternative splicing, giving rise to several mRNA species. MAPT transcripts are differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. MAPT gene mutations have been associated with several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy.
Purification Method
Affinity purification
Gene ID
4137
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from rat brain, using Phospho-Tau-S404 Rabbit mAb (CABP1378) at 1:5000 dilution. Rat brain cells were treated with λ-PP mixed solution (1ul) at 30℃ for 30 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Western blot analysis of lysates from Mouse brain using Phospho-Tau-S404 Rabbit mAb (CABP1378) at 1:5000 dilution. Mouse brain lysates were treated with λ-PP mixed solution (1ul) at 30℃ for 30 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 30 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
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