The Phospho-Tau-T231 Monoclonal Antibody (CABP0053) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced using rabbit monoclonal technology, is highly specific to the T231 phosphorylation site on the Tau protein, allowing for precise detection and analysis in various cell and tissue types.Tau protein plays a critical role in stabilizing microtubules in neurons, and abnormal phosphorylation of Tau has been linked to the formation of neurofibrillary tangles, a hallmark of Alzheimer's disease pathology.
This antibody is validated for use in ELISA, IF-P applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
Phospho-Tau-T231 Monoclonal Antibody
SKU:
CABP0053
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Clone Number:
ARC0021
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
VVRT PP
Tested Applications:
ELISAIF-P
Recommended Dilution:
IF-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
This gene encodes the microtubule-associated protein tau (MAPT) whose transcript undergoes complex, regulated alternative splicing, giving rise to several mRNA species. MAPT transcripts are differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. MAPT gene mutations have been associated with several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy.
Purification Method
Affinity purification
Gene ID
4137
RRID
AB_2863802
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Confocal imaging of paraffin-embedded Rat brain tissue using Phospho-Tau-T231 Rabbit mAb (CABP0053, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (CABS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
Confocal imaging of paraffin-embedded Mouse brain tissue using Phospho-Tau-T231 Rabbit mAb (CABP0053, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (CABS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
