Western blot analysis of various lysates, using Phospho-Tau-T181 antibody (CABP1324) at1:20000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The Phospho-Tau-T181 Monoclonal Antibody (CABP1324) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in a monoclonal configuration, is highly specific and sensitive for detecting phosphorylated Tau protein at the T181 site. It is validated for use in various applications, including immunohistochemistry and Western blotting.Phosphorylated Tau protein at the T181 site is a hallmark feature of neurofibrillary tangles in Alzheimer's disease, making it a key target for research into disease mechanisms and potential therapeutic interventions.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Phospho-Tau-T181 Monoclonal Antibody
SKU:
CABP1324
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC54951
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
PKTP P
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:2000 - 1:20000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
This gene encodes the microtubule-associated protein tau (MAPT) whose transcript undergoes complex, regulated alternative splicing, giving rise to several mRNA species. MAPT transcripts are differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. MAPT gene mutations have been associated with several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy.
Purification Method
Affinity purification
Gene ID
4137
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from 293T cells transfected with CDK5+CDK5R1+Tau and CDK5+CDK5R1+mutant Tau(T181/T217A) using Phospho-Tau-T181 Rabbit mAb (CABP1324) at1:20000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
