PANX2 Antibody is a premium polyclonal that offers outstanding performance and reliability for demanding research applications. Rigorously validated for ELISA, WB, IHC, IF, this antibody ensures consistent, reproducible results across multiple experimental platforms. Demonstrates excellent reactivity with Human, Mouse and Rat samples, providing researchers with confidence in cross-species compatibility. Conveniently packaged in 50μg format to meet your experimental needs. For optimal performance, store at -20°C or -80°C and maintains stability for 12 months. Backed by rigorous quality control testing to ensure superior performance in your critical research applications.
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Western Blot: Positive WB detected in: HepG2 whole cell lysate, K562 whole cell lysate, Rat liver tissue, Mouse liver tissue. All lanes: PANX2 antibody at 4.2 µg/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 75, 71, 56 kDa. Observed band size: 75 kDa.
IHC image of PACO66004 diluted at 1:400 and staining in paraffin-embedded human melanoma performed on a Leica Bond™ system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of HepG2 cells with PACO66004 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
