PCBP1 Rabbit Polyclonal Antibody (CAB1044)

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SKU:
CAB1044
  • Western blot analysis of extracts of various cell lines, using PCBP1 antibody (CAB1044) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
  • Immunohistochemistry of paraffin-embedded human stomach using PCBP1 antibody (CAB1044) at dilution of 1:100 (40x lens).
  • Immunofluorescence analysis of C6 cells using PCBP1 Rabbit pAb (CAB1044) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH-3T3 cells using PCBP1 Rabbit pAb (CAB1044) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U-2 OS cells using PCBP1 Rabbit pAb (CAB1044) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Description

Technical Manual

PCBP1 Rabbit Polyclonal Antibody

Product Name:PCBP1 Rabbit Polyclonal Antibody
Product SKU:CAB1044
Size:20uL, 50 uL
Host Species:Rabbit
Purification Method:Affinity purification
Isotype:IgG
Background:

This intronless gene is thought to have been generated by retrotransposition of a fully processed PCBP-2 mRNA. This gene and PCBP-2 have paralogues (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. The protein encoded by this gene appears to be multifunctional. It along with PCBP-2 and hnRNPK corresponds to the major cellular poly(rC)-binding protein. It contains three K-homologous (KH) domains which may be involved in RNA binding. This encoded protein together with PCBP-2 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promote poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability.

Immunogen:A synthetic peptide corresponding to a sequence within amino acids 100-200 of human PCBP1 (NP_006187.2).
Sequence:

LRLVVPATQCGSLIGKGGCKIKEIRESTGAQVQVAGDMLPNSTERAITIAGVPQSVTECVKQICLVMLETLSQSPQGRVMTIPYQPMPASSPVICAGGQDR

Gene ID:5093
Swiss Prot:Q15365
Synonyms:PCBP1; HEL-S-85; HNRPE1; HNRPX; hnRNP-E1; hnRNP-X
Calculated MW:37kDa
Observed MW:37kDa
Reactivity:Human, Mouse, Rat
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:200
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting Immunohistochemistry Immunofluorescence 
Positive Samples:NIH/3T3, HepG2, MCF7, Mouse liver, Rat liver
Cellular Location:Cytoplasm, Nucleus

Product Images

PCBP1 Rabbit Polyclonal Antibody (CAB1044)

Western blot analysis of extracts of various cell lines, using PCBP1 antibody (CAB1044) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.

PCBP1 Rabbit Polyclonal Antibody (CAB1044)

Immunohistochemistry of paraffin-embedded human stomach using PCBP1 antibody (CAB1044) at dilution of 1:100 (40x lens).

PCBP1 Rabbit Polyclonal Antibody (CAB1044)

Immunofluorescence analysis of C6 cells using PCBP1 Rabbit pAb (CAB1044) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

PCBP1 Rabbit Polyclonal Antibody (CAB1044)

Immunofluorescence analysis of NIH-3T3 cells using PCBP1 Rabbit pAb (CAB1044) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

PCBP1 Rabbit Polyclonal Antibody (CAB1044)

Immunofluorescence analysis of U-2 OS cells using PCBP1 Rabbit pAb (CAB1044) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Uniprot Information

UniProt Protein Function:hnRNP E1: Single-stranded nucleic acid binding protein that binds preferentially to oligo dC. Abundantly expressed in skeletal muscle, thymus and peripheral blood leukocytes while a lower expression is observed in prostate, spleen, testis, ovary, small intestine, heart, liver, adrenal and thyroid glands.
UniProt Protein Details:

Protein type:Translation; RNA splicing; RNA-binding

Chromosomal Location of Human Ortholog: 2p13-p12

Cellular Component: cytoplasm; intracellular membrane-bound organelle; membrane; nucleoplasm

Molecular Function:mRNA binding; protein binding; RNA binding; single-stranded DNA binding

Biological Process: gene expression; nuclear mRNA splicing, via spliceosome

NCBI Summary:This intronless gene is thought to have been generated by retrotransposition of a fully processed PCBP-2 mRNA. This gene and PCBP-2 have paralogues (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. The protein encoded by this gene appears to be multifunctional. It along with PCBP-2 and hnRNPK corresponds to the major cellular poly(rC)-binding protein. It contains three K-homologous (KH) domains which may be involved in RNA binding. This encoded protein together with PCBP-2 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promote poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. [provided by RefSeq, Jul 2008]
UniProt Code:Q15365
NCBI GenInfo Identifier:42560548
NCBI Gene ID:5093
NCBI Accession:Q15365.2
UniProt Secondary Accession:Q15365,Q13157, Q14975,
UniProt Related Accession:Q15365
Molecular Weight:37,498 Da
NCBI Full Name:Poly(rC)-binding protein 1
NCBI Synonym Full Names:poly(rC) binding protein 1
NCBI Official Symbol:PCBP1 
NCBI Official Synonym Symbols:HNRPX; HNRPE1; hnRNP-X; HEL-S-85; hnRNP-E1 
NCBI Protein Information:poly(rC)-binding protein 1
UniProt Protein Name:Poly(rC)-binding protein 1
UniProt Synonym Protein Names:Alpha-CP1; Heterogeneous nuclear ribonucleoprotein E1; hnRNP E1; Nucleic acid-binding protein SUB2.3
Protein Family:Poly(rC)-binding protein
UniProt Gene Name:PCBP1 
UniProt Entry Name:PCBP1_HUMAN
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Additional Information

Product type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
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