Anti-PDIA6 Antibody (CAB7055)

Antibody Name:	Anti-PDIA6 Antibody
Antibody SKU:	CAB7055
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 20-300 of human PDIA6 (NP_005733.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	HL-60, SW480, HepG2, THP-1, Mouse liver, Mouse lung, Mouse ovary, Mouse testis, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 20-300 of human PDIA6 (NP_005733.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LYSS SDDV IELT PSNF NREV IQSD SLWL VEFY APWC GHCQ RLTP EWKK AATA LKDV VKVG AVDA DKHH SLGG QYGV QGFP TIKI FGSN KNRP EDYQ GGRT GEAI VDAA LSAL RQLV KDRL GGRS GGYS SGKQ GRSD SSSK KDVI ELTD DSFD KNVL DSED VWMV EFYA PWCG HCKN LEPE WAAA ASEV KEQT KGKV KLAA VDAT VNQV LASR YGIR GFPT IKIF QKGE SPVD YDGG RTRS DIVS RALD LFSD NAPP PELL EIIN EDIA KRTC EEHQ LCVV A
Gene ID:	10130
Uniprot:	Q15084
Cellular Location:	Cell membrane, Endoplasmic reticulum lumen, Melanosome
Calculated MW:	47kDa/48kDa/53kDa
Observed MW:	48kDa

Synonyms:

PDIA6, ERP5, P5, TXNDC7

Background:

This gene encodes a member of the disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins that catalyze protein folding and thiol-disulfide interchange reactions. The encoded protein has an N-terminal ER-signal sequence, two catalytically active thioredoxin (TRX) domains, a TRX-like domain, and a C-terminal ER-retention sequence. This protein inhibits the aggregation of misfolded proteins and exhibits both isomerase and chaperone activity. Alternative splicing results in multiple transcript variants encoding different isoforms.

UniProt Protein Function:	PDIA6: May function as a chaperone that inhibits aggregation of misfolded proteins. Plays a role in platelet aggregation and activation by agonists such as convulxin, collagen and thrombin. Belongs to the protein disulfide isomerase family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:EC 5.3.4.1; Isomerase Chromosomal Location of Human Ortholog: 2p25.1 Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum lumen; endoplasmic reticulum; melanosome; plasma membrane; ER-Golgi intermediate compartment Molecular Function:protein binding; protein disulfide isomerase activity Biological Process: unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; protein folding; unfolded protein response; cell redox homeostasis; apoptotic cell clearance
NCBI Summary:	Protein disulfide isomerases (EC 5.3.4.1), such as PDIA6, are endoplasmic reticulum (ER) resident proteins that catalyze formation, reduction, and isomerization of disulfide bonds in proteins and are thought to play a role in folding of disulfide-bonded proteins (Hayano and Kikuchi, 1995 [PubMed 7590364]).[supplied by OMIM, Mar 2008]
UniProt Code:	Q15084
NCBI GenInfo Identifier:	2501205
NCBI Gene ID:	10130
NCBI Accession:	Q15084.1
UniProt Secondary Accession:	Q15084,Q53RC7, Q6ZSH5, Q99778, B3KY95, B5MCQ5, B7Z254 B7Z4M8, F8WA83,
UniProt Related Accession:	Q15084
Molecular Weight:	440
NCBI Full Name:	Protein disulfide-isomerase A6
NCBI Synonym Full Names:	protein disulfide isomerase family A, member 6
NCBI Official Symbol:	PDIA6
NCBI Official Synonym Symbols:	P5; ERP5; TXNDC7
NCBI Protein Information:	protein disulfide-isomerase A6; ER protein 5; protein disulfide isomerase P5; endoplasmic reticulum protein 5; thioredoxin domain-containing protein 7; protein disulfide isomerase-associated 6; protein disulfide isomerase-related protein; thioredoxin doma
UniProt Protein Name:	Protein disulfide-isomerase A6
UniProt Synonym Protein Names:	Endoplasmic reticulum protein 5; ER protein 5; ERp5; Protein disulfide isomerase P5; Thioredoxin domain-containing protein 7
Protein Family:	Protein disulfide-isomerase
UniProt Gene Name:	PDIA6
UniProt Entry Name:	PDIA6_HUMAN

	Western blot analysis of extracts of various cell lines, using PDIA6 antibody (CAB7055) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunohistochemistry of paraffin-embedded rat brain using PDIA6 Antibody (CAB7055) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using PDIA6 Antibody (CAB7055) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using PDIA6 antibody (CAB7055). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-PDIA6 Antibody (CAB18092)[KO Validated]	Human Protein Disulfide Isomerase A6 / PDIA6 ELISA Kit
Anti-PDIA6 Antibody (CAB4260)	Human PDIA6 (Protein Disulfide Isomerase A6) CLIA Kit (HUES00888)
PDIA6 Antibody (PACO11142)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)