The PDLIM1/CLP36 Antibody (CAB6686) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, specifically detects PDLIM1 in human samples and is validated for use in Western blot applications.PDLIM1, also known as CLP-36, is a PDZ-LIM domain protein that regulates the function of other proteins through interactions with their PDZ-binding motifs. It plays a crucial role in organizing signaling complexes and modulating cellular pathways involved in cell adhesion, migration, and differentiation.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
PDLIM1/CLP36 Antibody
SKU:
CAB6686
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
This gene encodes a member of the enigma protein family. The protein contains two protein interacting domains, a PDZ domain at the amino terminal end and one to three LIM domains at the carboxyl terminal. It is a cytoplasmic protein associated with the cytoskeleton. The protein may function as an adapter to bring other LIM-interacting proteins to the cytoskeleton. Pseudogenes associated with this gene are located on chromosomes 3, 14 and 17.
Purification Method
Affinity purification
Gene ID
9124
RRID
AB_2767270
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using PDLIM1/CLP36 Rabbit pAb (CAB6686) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunofluorescence analysis of U2OS cells using PDLIM1/CLP36 Rabbit pAb (CAB6686). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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