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PerCP/Cyanine5.5 Anti-Mouse Ly6A/E (Sca-1) Antibody [D7] (AGEL2351)

Datasheet MSDS
SKU AGEL2351
Product Type Antibody
Antibody Type Monoclonal Antibody
Applications FCM
Reactivity Mouse
Host Species Rat
Isotype Rat IgG2a, κ
Clone D7
Conjugation PerCP/Cyanine5.5
€75 - €203

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Description

system_update_altDatasheetsystem_update_altMSDS

PerCP/Cyanine5.5 Anti-Mouse Ly6A/E (Sca-1) Antibody [D7]

The PE/Cy5.5 Anti-Mouse LY6A/E (Sca-1) Antibody (D7) is a crucial tool for researchers studying mouse immune cells and stem cell biology. This antibody, offered by Assay Genie, is specific for LY6A/E (Sca-1) antigen, a cell surface marker commonly used to identify hematopoietic stem cells and other progenitor cells in various tissues.The D7 antibody is conjugated to PE/Cy5.5, a fluorescent dye that facilitates multi-color flow cytometry experiments for accurate cell identification and sorting. Its high specificity and low background staining make it an ideal choice for applications such as flow cytometry analysis, immunofluorescence staining, and cell sorting.LY6A/E (Sca-1) is a well-established marker for identifying mouse hematopoietic stem cells, which have the potential to differentiate into various blood cell types.

By targeting this antigen with the D7 antibody, researchers can investigate the presence and characteristics of stem cells in different mouse tissues and explore their roles in tissue regeneration, immune responses, and disease development.In conclusion, the PE/Cy5.5 Anti-Mouse LY6A/E (Sca-1) Antibody (D7) from Assay Genie offers reliable performance and specificity for researchers studying mouse stem cells and immune cell populations. Its use can advance our understanding of stem cell biology, immune system function, and disease mechanisms in preclinical research settings.

Product Title:PerCP/Cyanine5.5 Anti-Mouse Ly6A/E (Sca-1) Antibody [D7]
SKU:AGEL2351
Size:50 Tests, 100 Tests, 200 Tests
Clone No:D7
Synonyms:Ly6a;Ly6;Ly-6A.2/Ly-6E.1;SCA-1;TAP;
Host Species:Rat
Reactivity:Mouse
Application:FCM
Isotype:Rat IgG2a, κ
Isotype Control:PerCP/Cyanine5.5 Rat IgG2a, κ Isotype Control[2A3]
Form:Liquid
Conjugation:PerCP/Cyanine 5.5
Recommended Use:Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Spectrum:Spectrum Image
Swissprot:P05533
Gene ID:110454
Storage Buffer:Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:Ly-6A/E, also known as Sca-1, is an 18 kD member of the Ly-6 multigene family. Ly6A/E is a glycosylphosphatidylinositol (GPI)-linked protein expressed on hematopoietic stem cells. In mice expressing the Ly-6.2 haplotype (e.g., AKR, C57BL, C57BR, DBA/2, SJL, SWR, and 129), Ly-6A/E is also expressed on peripheral B lymphocytes and thymic and peripheral T lymphocytes. Strains expressing the Ly-6.1 haplotype (e.g., BALB/c, CBA, C3H/He, DBA/1, and NZB) have low Ly-6A/E expression on resting peripheral lymphocytes. The expression of Ly-6A/E on lymphocytes is upregulated upon activation from both Ly6.1 and Ly6.2 haplotype mice. Ly-6A/E is thought to be involved in the regulation of both T and B cell responses.
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