Anti-PRPH Antibody (CAB4048)

Antibody Name:	Anti-PRPH Antibody
Antibody SKU:	CAB4048
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human PRPH (NP_006253.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:20 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-251MG, HepG2, MCF7, Mouse brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human PRPH (NP_006253.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSHH PSGL RAGF SSTS YRRT FGPP PSLS PGAF SYSS SSRF SSSR LLGS ASPS SSVR LGSF RSPR AGAG ALLR LPSE RLDF SMAE ALNQ EFLA TRSN EKQE LQEL NDRF ANFI EKVR FLEQ QNAA LRGE LSQA RGQE PARA DQLC QQEL RELR RELE LLGR ERDR VQVE RDGL AEDL AALK QRLE EETR KRED AEHN LVLF RKDV DDAT LSRL ELER KIES LMDE IEFL KKLH EEEL RDLQ VSVE SQQV QQVE VEAT VKPE
Gene ID:	5630
Uniprot:	P41219
Cellular Location:
Calculated MW:	53kDa
Observed MW:	54-62kDa

Synonyms:	PRPH, NEF4, PRPH1
Background:	This gene encodes a cytoskeletal protein found in neurons of the peripheral nervous system. The encoded protein is a type III intermediate filament protein with homology to other cytoskeletal proteins such as desmin, and is a different protein that the peripherin found in photoreceptors. Mutations in this gene have been associated with susceptibility to amyotrophic lateral sclerosis.

UniProt Protein Function:	peripherin: Class-III neuronal intermediate filament protein. Belongs to the intermediate filament family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis; Cytoskeletal Chromosomal Location of Human Ortholog: 12q12-q13 Cellular Component: membrane; type III intermediate filament; neurofilament; intermediate filament Molecular Function:structural molecule activity Biological Process: intermediate filament cytoskeleton organization and biogenesis Disease: Amyotrophic Lateral Sclerosis 1
NCBI Summary:	This gene encodes a cytoskeletal protein found in neurons of the peripheral nervous system. The encoded protein is a type III intermediate filament protein with homology to other cytoskeletal proteins such as desmin, and is a different protein that the peripherin found in photoreceptors. Mutations in this gene have been associated with susceptibility to amyotrophic lateral sclerosis. [provided by RefSeq, Jul 2008]
UniProt Code:	P41219
NCBI GenInfo Identifier:	118585871
NCBI Gene ID:	5630
NCBI Accession:	P41219.2
UniProt Secondary Accession:	P41219,Q8N577,
UniProt Related Accession:	P41219
Molecular Weight:	470
NCBI Full Name:	Peripherin
NCBI Synonym Full Names:	peripherin
NCBI Official Symbol:	PRPH
NCBI Official Synonym Symbols:	NEF4; PRPH1
NCBI Protein Information:	peripherin; neurofilament 4 (57kD)
UniProt Protein Name:	Peripherin
UniProt Synonym Protein Names:	Neurofilament 4
Protein Family:	Peripherin
UniProt Gene Name:	PRPH
UniProt Entry Name:	PERI_HUMAN

	Western blot analysis of extracts of various cell lines, using PRPH antibody (CAB4048) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat heart using PRPH antibody (CAB4048) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse heart using PRPH antibody (CAB4048) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
PRPH Antibody (PACO06777)	Peripherin Colorimetric Cell-Based ELISA Kit
PRPH Antibody (PACO11501)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)