The Peroxiredoxin 4 (PRDX4) Monoclonal Antibody (CAB9131) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, derived from rabbit monoclonal sources, demonstrates high specificity and sensitivity when detecting Peroxiredoxin-4 in human samples through techniques such as Western blotting.Peroxiredoxin-4 is known for its role in protecting cells from oxidative stress and maintaining cellular homeostasis. Its function in regulating redox signaling pathways has garnered interest in its implications for various diseases, including cancer, neurodegenerative disorders, and cardiovascular conditions.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Peroxiredoxin 4 (PRDX4) Monoclonal Antibody
SKU:
CAB9131
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1442
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
The protein encoded by this gene is an antioxidant enzyme and belongs to the peroxiredoxin family. The protein is localized to the cytoplasm. Peroxidases of the peroxiredoxin family reduce hydrogen peroxide and alkyl hydroperoxides to water and alcohol with the use of reducing equivalents derived from thiol-containing donor molecules. This protein has been found to play a regulatory role in the activation of the transcription factor NF-kappaB.
Purification Method
Affinity purification
Gene ID
10549
RRID
AB_2863664
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Peroxiredoxin 4 (PRDX4) Rabbit mAb (CAB9131) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using Peroxiredoxin 4 (PRDX4) Rabbit mAb (CAB9131) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using Peroxiredoxin 4 (PRDX4) Rabbit mAb (CAB9131) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using Peroxiredoxin 4 (PRDX4) Rabbit mAb (CAB9131) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human liver tissue using Peroxiredoxin 4 (PRDX4) Rabbit mAb (CAB9131) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using Peroxiredoxin 4 (PRDX4) Rabbit mAb (CAB9131) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
