Anti-PFKFB3 Antibody (CAB6945)

Antibody Name:	Anti-PFKFB3 Antibody
Antibody SKU:	CAB6945
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 400-500 of human PFKFB3 (NP_004557.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:1000 IHC 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, SKOV3

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 400-500 of human PFKFB3 (NP_004557.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LDKS AEEM PYLK CPLH TVLK LTPV AYGC RVES IYLN VESV CTHR ERSE DAKK GPNP LMRR NSVT PLAS PEPT KKPR INSF EEHV ASTS AALP SCLP PEVP T
Gene ID:	5209
Uniprot:	Q16875
Cellular Location:
Calculated MW:	57kDa/58kDa/59kDa/60kDa
Observed MW:	57kDa

Synonyms:

PFKFB3, IPFK2, PFK2, iPFK-2

Background:

The protein encoded by this gene belongs to a family of bifunctional proteins that are involved in both the synthesis and degradation of fructose-2, 6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. The encoded protein has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2, 6-bisphosphate (F2, 6BP), and a fructose-2, 6-biphosphatase activity that catalyzes the degradation of F2, 6BP. This protein is required for cell cycle progression and prevention of apoptosis. It functions as a regulator of cyclin-dependent kinase 1, linking glucose metabolism to cell proliferation and survival in tumor cells. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	PFKFB3: Synthesis and degradation of fructose 2,6-bisphosphate. Ubiquitous. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Kinase, other; Motility/polarity/chemotaxis; Carbohydrate Metabolism - fructose and mannose; EC 3.1.3.46; Phosphatase (non-protein); EC 2.7.1.105 Chromosomal Location of Human Ortholog: 10p15.1 Cellular Component: cytosol; nucleoplasm Molecular Function:6-phosphofructo-2-kinase activity
NCBI Summary:	The protein encoded by this gene belongs to a family of bifunctional proteins that are involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. The encoded protein has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2,6-bisphosphate (F2,6BP), and a fructose-2,6-biphosphatase activity that catalyzes the degradation of F2,6BP. This protein is required for cell cycle progression and prevention of apoptosis. It functions as a regulator of cyclin-dependent kinase 1, linking glucose metabolism to cell proliferation and survival in tumor cells. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2016]
UniProt Code:	Q16875
NCBI GenInfo Identifier:	3023733
NCBI Gene ID:	5209
NCBI Accession:	Q16875.1
UniProt Secondary Accession:	Q16875,O43622, O75902, Q5VX15, Q5VX18, Q5VX19, B7Z955
UniProt Related Accession:	Q16875
Molecular Weight:	60,602 Da
NCBI Full Name:	6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3
NCBI Synonym Full Names:	6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3
NCBI Official Symbol:	PFKFB3
NCBI Official Synonym Symbols:	PFK2; IPFK2; iPFK-2
NCBI Protein Information:	6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3
UniProt Protein Name:	6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3
UniProt Synonym Protein Names:	6PF-2-K/Fru-2,6-P2ase brain/placenta-type isozyme; Renal carcinoma antigen NY-REN-56; iPFK-2Including the following 2 domains:6-phosphofructo-2-kinase (EC:2.7.1.105)Fructose-2,6-bisphosphatase (EC:3.1.3.46)
Protein Family:	6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase
UniProt Gene Name:	PFKFB3
UniProt Entry Name:	F263_HUMAN

	Western blot analysis of extracts of various cell lines, using PFKFB3 antibody (CAB6945) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded rat ovary using PFKFB3 Rabbit pAb (CAB6945) at dilution of 1:25 (40x lens).
	Immunohistochemistry of paraffin-embedded human breast cancer using PFKFB3 Rabbit pAb (CAB6945) at dilution of 1:25 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse stomach using PFKFB3 Rabbit pAb (CAB6945) at dilution of 1:25 (40x lens).

Antibodies	ELISA Kits
Anti-PFKFB3 Antibody (CAB14764)	Human PFKFB3 (6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3) ELISA Kit (HUFI07256)
Anti-PFKFB3 Antibody (CAB3934)
PFKFB3 Antibody (PACO11184)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)