The PGA5 Antibody (CAB12951) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high specificity and reactivity with human samples, making it ideal for use in Western blot applications. By targeting the PGA5 protein, this antibody enables the detection and analysis of PGA5 expression in various cell types, providing insights into its role in cellular processes and disease pathways.PGA5, a key player in the ubiquitin-proteasome system, is critical for maintaining protein homeostasis and regulating cell function.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
PGA5 Antibody
SKU:
CAB12951
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
Pg5, PGA5
Positive Sample:
U-87MG, HT-29, 293T, Mouse brain, Mouse stomach, Rat brain
Cellular Localization:
Secreted.
Calculated MW:
42kDa
Observed MW:
42kDa
This gene encodes a protein precursor of the digestive enzyme pepsin, a member of the peptidase A1 family of endopeptidases. The encoded precursor is secreted by gastric chief cells and undergoes autocatalytic cleavage in acidic conditions to form the active enzyme, which functions in the digestion of dietary proteins. This gene is found in a cluster of related genes on chromosome 11, each of which encodes one of multiple pepsinogens. Pepsinogen levels in serum may serve as a biomarker for atrophic gastritis and gastric cancer.
Purification Method
Affinity purification
Gene ID
5222
RRID
AB_2759797
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using PGA5 Rabbit pAb (CAB12951) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
