Anti-PHIP Antibody (CAB7207)

Antibody Name:	Anti-PHIP Antibody
Antibody SKU:	CAB7207
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1542-1821 of human PHIP (NP_060404.3).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	HeLa, Jurkat

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1542-1821 of human PHIP (NP_060404.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	KTIL ENSV KHSK ALNT LSSP GQSS FSHG TRNN SAKE NMEK EKPV KRKM KSSV LPKA STLS KSSA VIEQ GDCK NNAL VPGT IQVN GHGG QPSK LVKR GPGR KPKV EVNT NSGE IIHK KRGR KPKK LQYA KPED LEQN NVHP IRDE VLPS STCN FLSE TNNV KEDL LQKK NRGG RKPK RKMK TQKL DADL LVPA SVKV LRRS NRKK IDDP IDEE EEFE ELKG SEPH MRTR NQGR RTAF YNED DSEE EQRQ LLFE DTSL TFGT SSRG RVRK LTEK AKAN LIGW
Gene ID:	55023
Uniprot:	Q8WWQ0
Cellular Location:	Nucleus
Calculated MW:	206kDa
Observed MW:	250kDa

Synonyms:	PHIP, BRWD2, DCAF14, WDR11, ndrp
Background:

UniProt Protein Function:	PHIP: Probable regulator of the insulin and insulin-like growth factor signaling pathways. Stimulates cell proliferation through regulation of cyclin transcription and has an anti- apoptotic activity through AKT1 phosphorylation and activation. Plays a role in the regulation of cell morphology and cytoskeletal organization. Interacts with IRS1 and IRS2. Interacts (via bromo domain) with acetylated lysine residues on histone H1.4, histone H3 and H4 (in vitro)
UniProt Protein Details:	Chromosomal Location of Human Ortholog: 6q14 Cellular Component: nucleus Molecular Function:protein binding; insulin receptor binding Biological Process: positive regulation of insulin-like growth factor receptor signaling pathway; regulation of cell shape; regulation of protein amino acid phosphorylation; positive regulation of mitosis; protein import into nucleus; regulation of growth; positive regulation of transcription, DNA-dependent; positive regulation of cell proliferation; insulin receptor signaling pathway; regulation of cell morphogenesis; cytoskeleton organization and biogenesis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of peptidyl-serine phosphorylation; negative regulation of apoptosis
NCBI Summary:	PHIP binds the pleckstrin homology (PH) domain of insulin receptor substrate-1 (IRS1; MIM 147545), modulates insulin signaling, and plays a role in pancreatic beta cell growth and survival (Farhang-Fallah et al., 2000 [PubMed 11018022]; Podcheko et al., 2007 [PubMed 17636024]).[supplied by OMIM, Jun 2009]
UniProt Code:	Q8WWQ0
NCBI GenInfo Identifier:	308153472
NCBI Gene ID:	55023
NCBI Accession:	Q8WWQ0.2
UniProt Secondary Accession:	Q8WWQ0,Q05CQ9, Q5VVH4, Q66I29, Q69YV1, Q8NBZ5, Q96H52 Q96ME2, Q9H261, A7J992, B2RPK4,
UniProt Related Accession:	Q8WWQ0
Molecular Weight:	1821
NCBI Full Name:	PH-interacting protein
NCBI Synonym Full Names:	pleckstrin homology domain interacting protein
NCBI Official Symbol:	PHIP
NCBI Official Synonym Symbols:	ndrp; BRWD2; WDR11; DCAF14
NCBI Protein Information:	PH-interacting protein; IRS-1 PH domain-binding protein; WD repeat-containing protein 11; DDB1 and CUL4 associated factor 14
UniProt Protein Name:	PH-interacting protein
UniProt Synonym Protein Names:	IRS-1 PH domain-binding protein; WD repeat-containing protein 11
Protein Family:	PH-interacting protein
UniProt Gene Name:	PHIP
UniProt Entry Name:	PHIP_HUMAN

	Western blot analysis of extracts of various cell lines, using PHIP antibody (CAB7207) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human well-differentiated squamous skin carcinoma using PHIP antibody (CAB7207) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human kidney cancer using PHIP antibody (CAB7207) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse tumor using PHIP antibody (CAB7207) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of MCF-7 cells using PHIP antibody (CAB7207).

Antibodies

PHIP Antibody (PACO11215)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)