The Phospho-CDC25C (S216) Polyclonal Antibody (CABP0577) is an essential tool for researchers studying the phosphorylation of CDC25C at serine 216, a key regulatory event in cell cycle progression. This antibody, produced in rabbits, exhibits high specificity and sensitivity towards human samples, making it ideal for use in Western blot applications.CDC25C is a critical regulator of cell cycle progression, functioning as a phosphatase that activates cyclin-dependent kinases. Phosphorylation of CDC25C at serine 216 is known to play a significant role in controlling the activity of CDC25C and therefore modulating cell cycle progression.
Research involving this phosphorylation event is crucial for understanding cell cycle regulation and its dysregulation in diseases such as cancer.This antibody enables accurate detection and analysis of phosphorylated CDC25C (S216) in various cell types, providing valuable insights into the mechanisms underlying cell cycle control. By studying the phosphorylation status of CDC25C, researchers can further elucidate its role in normal cell growth and proliferation, as well as its potential implications in disease pathogenesis.
Product Name:
Phospho-cdc25C-S216 Rabbit Polyclonal Antibody
SKU:
CABP0577
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S216 of human cdc25C (NP_001781.2).
Sequence:
SPSM P
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB,1:100 - 1:500 IHC-P,1:50 - 1:200
Synonyms:
CDC25; PPP1R60; Phospho-cdc25C-S216
Positive Sample:
HT-29+λ-PP
Conjugate:
Unconjugated
Cellular Localization:
Nucleus.
Calculated MW:
53kDa
Observed MW:
60kDa
This gene encodes a conserved protein that plays a key role in the regulation of cell division. The encoded protein directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It also suppresses p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described.
Purification Method:
Affinity purification
Gene ID:
995
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HT-29 cells, using Phospho-cdc25C-S216 Rabbit pAb (CABP0577) at 1:400 dilution.HT-29 cells were treated by λ-PP mixed solution (1ul) at 30℃ for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
