Western blot analysis of HeLa, using Phospho-GCN2-T899 Rabbit pAb (CABP1428) at 1:1000 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The Phospho-GCN2-T899 Polyclonal Antibody (CABP1428) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, demonstrates high reactivity with human samples and is specifically validated for use in Western blot applications. By targeting the phosphorylated form of GCN2 at threonine 899, this antibody allows for the precise detection and quantification of this important protein in various cell types.GCN2, also known as General Control Non-derepressible 2, is a key player in the integrated stress response pathway that helps cells adapt to environmental stressors such as nutrient deprivation. Phosphorylation of GCN2 at threonine 899 is a crucial event in its activation and signaling cascade.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Phospho-GCN2-T899 Polyclonal Antibody
SKU:
CABP1428
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
HLTG M
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
GCN2, PVOD2, Phospho-GCN2-T899
Positive Sample:
HeLa treated with Calyculin A
Cellular Localization:
Cytosol, Cytosolic Ribosome.
Calculated MW:
187kDa
Observed MW:
187kDa
This gene encodes a member of a family of kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor-2 (EIF2), resulting in the downregulaton of protein synthesis. The encoded protein responds to amino acid deprivation by binding uncharged transfer RNAs. It may also be activated by glucose deprivation and viral infection. Mutations in this gene have been found in individuals suffering from autosomal recessive pulmonary venoocclusive-disease-2.
Purification Method
Affinity purification
Gene ID
440275
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from HeLa cells, using Phospho-GCN2-T899 Rabbit pAb (CABP1428) at 1:1000 dilution. HeLa cells were treated with Calyculin A (100 nM) at 37℃ for 30 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
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